Full Description
This volume focuses on protein analysis, including a wide range of the use of mass spectrometry and other protein methods within neurobiological disciplines. Chapters cover topics such as cerebrospinal fluid (CSF) processing and biobanking; label-free quantitative proteomics; SWATH; top-down proteomics; and experimental strategies based on other -omics applied to CSF metabolome, lipidome, and microRNAome. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and thorough, Cerebrospinal Fluid (CSF) Proteomics: Methods and Protocols is a valuable resource for graduate students and post-doctoral fellows interested in learning more about CSF proteotyping. It is also useful to established researchers seeking furtherinsight into this growing field.
Contents
The Human Brain Proteome Project: Biological and Technological Challenges.- Guidelines for CSF Processing and Biobanking: Impact on the Identification and Development of Optimal CSF Protein Biomarkers.- Functional Analyses of Embryonic Cerebrospinal Fluid Proteins.- CSF Sample Preparation for Data Independent Acquisition.- Sample Fractionation Techniques for CSF Peptide Spectral Library Generation.- Application of 2D-DIGE and iTRAQ Workflows to Analyse CSF in Gliomas.- Peptidomic Workflow Applied to Cerebrospinal Fluid Analysis.- Quantitative Evaluation of Different Protein Fractions of Cerebrospinal Fluid using 18O Labeling.- A Versatile Workflow for Cerebrospinal Fluid Proteomic Analysis with Mass Spectrometry: A Matter of Choice between Deep Coverage and Sample Throughput.- Determination of Cerebrospinal Fluid Proteome Variations by Isobaric-Labeling Coupled to Strong Cation Exchange Chromatography and Tandem Mass-Spectrometry.- SWATH Mass Spectrometry Applied to Cerebrospinal Fluid Differential Proteomics: Establishment of a Sample-Specific Method.- Top-Down Proteomics Applied to Human CSF.- Application of an Aptamer-Based Proteomics Assay (SOMAscanTM) in Rat CSF.- Monitoring the Cerebrospinal Fluid Cytokine Profile using Membrane-Based Antibody Arrays.- Selective and Sensitive Mass Spectrometric Identification of Immune Complexe Antigens in Cerebrospinal Fluid.- CSF N-glycoproteomics using MALDI MS Techniques in Neurodegenerative Diseases.- Deployment of Label-Free Quantitative Olfactory Proteomics to Detect Cerebrospinal Fluid Biomarker Candidates in Synucleinopathies.- An Improved Assay for Quantitation of Cerebrospinal Fluid Cystatin C using Liquid Chromatography Tandem Mass Spectrometry.- Array-Based Profiling of Proteins and Autoantibody Repertoires in CSF.- Untargeted Metabolomics Relative Quantification by SWATH Mass Spectrometry Applied to Cerebrospinal Fluid.- Co-Extraction for Metabolomics and Proteomics from a Single CSF Sample.- Characterization of the Alzheimer's Disease MicroRNA Profile in Exosome Enriched CSF Samples.- Mass-Spectrometry Applied to Human Cerebrospinal Fluid Lipidome.- Trait Loci Mapping and CSF Proteome.- Essential Features and Use Cases of the Cerebrospinal Fluid Proteome Resource (CSF-PR).- Bioinformatics to Tackle the Biological Meaning of Human Cerebrospinal Fluid Proteome.
