Full Description
This second edition volume expands on the previous edition with new insights into the molecular mechanisms involved in allergy through the use of various protocols that cover disciplines like allergy, immunology, cell biology, and histology. The chapters in this book cover topics such as characterization of allergens using western blotting and mass spectrometry, collecting and sampling airborne allergens, IgG antibodies, identification and characterization of allergen specific T cells, and the role of antigen presenting cells in T cell proliferation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and thorough, Allergy: Methods and Protocols, Second Edition is a valuable resource for researchers interested in beginning or continuing their research in the study of allergies.
Contents
Understanding of the Molecular Mechanisms of Allergy.- T-Cell: Primary Culture from Peripheral Blood.- Measurement of Allergen-Specific Inhibitory Antibody Activity.- Immunoelectrophoresis for the Characterization of Allergen Extracts.- Standardization of Allergen Extracts.- Quantifying Human Innate Cytokine and Chemokine Responses Ex Vivo via Pattern Recognition Receptor Stimulation.- Improved Methods for Quantifying Human Chemokine and Cytokine Biomarker Responses: Ultrasensitive ELISA and Meso Scale Electrochemiluminescence Assays.- Development of Mould Antigen-Specific Enzyme-Linked Immunosorbent Assays (ELISA) to Quantify Airborne Antigen Exposure.- Monocyte-Derived Dendritic Cells as Antigen Presenting Cells in T-Cell Proliferation and Cytokine Production.- The Use of Dual Cell Tracker Dye Staining for the Identification and Characterization of Peanut Specific T-Cell Subsets.- The Use of pMHCII Tetramer Technology for the Identification and Characterization of CD4+ T Cells.- Isolation and Characterization of Lymphocytes from Human Mucosal Biopsies.- Development of a Dipstick Assay for Soy Allergens: Inexpensive Detection to Control Allergen Exposure.- Novel Method for the Purification of House Dust Mite Allergen Der p 1 and Its Use in Structure-Based Chemical Design of Novel Inhibitors.- Measurement of Specific IgG Anti-Fel d 1 Antibodies.- Measurement of Specific IgG4 Anti-Mouse Urine Antibodies.- Preparation of Samples for a Mass Spectrometry Based Method to Identify Allergenic Proteins.
