Full Description
This volume details protocols on formulation, surface modification, characterization, and application of a variety of pharmaceutical nanocarriers such as micelles, nanoparticles, dendrimers, carbon dots, polymersomes, and others. Chapters are targeted toward investigators working in academic and industrial laboratories conducting research in the broad field of pharmaceutical sciences, with an emphasis on drug delivery. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Pharmaceutical Nanotechnology: Basic Protocols aims to be a source of inspiration to all investigators who are interested in the potential of the merger of nanotechnology with pharmaceutical sciences.
Contents
Nanovesicles for Nanomedicine: Theory and Practices.- Preparation and Characterization of Micelles.- Anionic and Cationic Vitamin E-TPGS Mixed Polymeric Phospholipid Micellar Vehicles.- Phospholipid Micelles for Peptide Drug Delivery.- Design, Preparation, and Characterization of Peptide-Based Nanocarrier for Gene Delivery.- Gelatin Nanoparticles.- Green Synthesis of Chitosan-Silver/Gold Hybrid Nanoparticles for Biomedical Applications.- Methods of Fabrication of Chitosan-Based Nano-in-Microparticles (NMPs).- Fabrication of Mucoadhesive-Dendrimers as Solid Dosage Forms.- In Situ Vaccination of Tumors Using Plant Viral Nanoparticles.- Bioconjugation in Drug Delivery: Practical Perspectives and Future Perceptions.- Conjugation of Triphenylphosphonium Cation to Hydrophobic Moieties to Prepare Mitochondria-Targeting Nanocarriers.- Surface Modification of Biomedically Essential Nanoparticles Employing Polymer Coating.- Peptide-Modified Gemini Surfactants: Preparation and Characterization for Gene Delivery.- Preparation of Responsive Carbon Dots for Anticancer Drug Delivery.- Surface Modification of Nanoparticles and Nanovesicles via Click Chemistry.- Polymersomes: Preparation and Characterization.- Partially Polymerized Phospholipid Vesicles for Efficient Delivery of Macromolecules.- Fabrication of Nanostructured Lipid Carriers (NLC)-Based Gels from Microemulsion Template for Delivery through Skin.- Preparation and Characterization of Solid Lipid Nanoparticles-Based Gel for Topical Delivery.- Molecular-Level "Observations" of the Behavior of Gold Nanoparticles in Aqueous Solution and Interacting with a Lipid Bilayer Membrane.- Atomic Force Microscopy for Cell Membrane Investigation.- Physico-Chemical Characterization of Phthalocyanine-Functionalized Quantum Dots by Capillary Electrophoresis Coupled to a LED Fluorescence Detector.- In Vitro Testing of Anticancer Nanotherapeutics Using Tumor Spheroids.
