Full Description
The volume presents a variety of protocols that will be useful to researchers working in the field of disease modeling, drug discovery, and the cell biology of various tissues. Chapters in this book cover topics such as requirements and preconditions for using human induced pluripotent cell lines in assay development; the culture of iPSCs and iPSC-derived cardiomyocytes by bioreactor technologies; distinctions between hepatocytes, vascular endothelial cells, cardiomyocytes, renal podocytes, and neurons; and high content assays of mitochondrial function and autophagy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Cell-Based Assay using iPSCs for Drug Development and Testing: Methods and Protocols is a valuable resource for researchers working in this important and developing field of study.
Contents
Cell-Based Assays using Differentiated Human Induced Pluripotent Cells.- Monolayer Generation of Vascular Endothelial Cells from Human Pluripotent Stem Cells.- Generating Human iPSC-Derived Astrocytes with Chemically Defined Medium for In Vitro Disease Modelling.- Human Induced Pluripotent Stem Cell-Derived Definitive Endoderm Bulk Culture and Hepatic Differentiation.- Production of Cardiomyocytes from Human Pluripotent Stem Cells by Bioreactor Technologies.- Improved Protocol of Cardiac Differentiation and Maturation of Pluripotent Stem Cells.- Human Pluripotent Stem Cell Expansion in Stirred Tank Bioreactors.- A Protocol for the One-Step Differentiation of Human Induced Pluripotent Stem Cells into Mature Podocytes.- Differentiation of hiPS Cells into Definitive Endoderm for High Throughput Screening.- LC-MS/MS Based Metabolomics for Cell Cultures.- Preparation of iPSCs for Targeted Proteomic Analysis.- Measurement of Bile Acids as a Marker of the Functionality of iPSC-Derived Hepatocytes.- Mass Spectrometry Measurement of Albumin/Alpha Fetoprotein Ratio as an Indicator of iPSC-Derived Hepatocyte Differentiation.- Seeding Induced Pluripotent Stem Cell-Derived Neurons onto 384-Well Plates.- High Content Autophagy Analysis in iPSC-Derived Neurons using Immuno-Fluorescence.- High Content Analysis of Mitochondrial Function in iPSC-Derived Neurons.- Evaluating the Effect of Drug Compounds on Cardiac Spheroids using the Cardiac Cell Outgrowth Assay.- Assay Procedures for Compound Testing of hiPSC-Derived Cardiomyocytes using Multi-Well Microelectrode Arrays.- Multielectrode Array (MEA) Based Detection of Spontaneous Network Activity in Human iPSC-Derived Cortical Neurons.- Assessing Neuronal Excitability on a Fluorometric Imaging Plate Reader (FLIPR) Following a Defined Electrostimulation Paradigm.- Fabrication of a Microfluidic Cell Culture Device using Photolithographic and Soft Lithographic Techniques.- Using a Microfluidic Device for Culture and Drug Toxicity Testing of 3D Cells.- hiPS Cell-Derived Neurons for High-Throughput Screening.
