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Full Description
This volume explores the scope of the cellular redox analysis and the importance of not being limited by frequently changing and evolving technology. The chapters in this book cover a wide range of topics such as redox components in animal and plant cells and the role of reactive oxygen species, reactive nitrogen species, and hydrogen sulphide in cell signaling; measuring modifications using Flow Cytometry, ELISA assays, and Western blot analysis; measurement of oxidative stress in mitochondira and biological systems; and the use of the genetically encoded fluorescent probe HyPer. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and informative, Redox-Mediated Signal Transduction: Methods and Protocols, Second Edition is a valuable resource for both novice and expert researchers who want to expand their studies into new areas and new systems in the evolving redox field.
Contents
The Role of Redox in Signal Transduction.- Methods for the Addition of Redox Compounds.- Investigating ROS, RNS, and H2S Sensitive Signalling Proteins.- Measurement of 4-hydroxynonenal (4-HNE) Protein Adducts by ELISA.- Using Flow Cytometry to Detect and Measure Intracellular Thiol Redox Status in Viable T Cells from Heterogeneous Populations.- Detection of S-Nitrosation and S-Glutathionylation of the Human Branched-Chain Aminotransferase Proteins.- Visualization of Intracellular Hydrogen Peroxide produced by DAO with Genetically Encoded Fluorescent Probe HyPer in NIH-3T3 Cells.- Applications of Electron Paramagnetic Resonance (EPR) Spectroscopy to the Study of Oxidative Stress in Biological Systems.- Extracellular and Intracellular NO Detection in Plants by Diaminofluoresceins.- Working with Hypoxia.- Predicting the Effects of Low Dose-Rate Ionising Radiation on Redox Potential in Plant Cells.- Thioredoxin-1 PEGyletion as an In Vitro Method for Drug Target Identification.- Redox-Regulated, Targeted Affinity Isolation of NADH-Dependent Protein Interactions with the Branched Chain Aminotransferase Proteins.- Analysis of Redox Relationships in the Plant Cell Cycle: Determination of Ascorbate, Glutathione and Poly(ADPribose)polymerase (PARP) in Plant Cell Cultures.- Equations to Support Redox Experimentation.
