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Full Description
This volume expands on the previous edition with a more extensive look at molecular motors and their roles in muscle contractions, vesicle transport, flagellar beating, chromosome segregation, and DNA replication and repair. The chapters in this book are divided into three parts: Part One looks at membrane motors, such as the bacterial flagellar rotary motor; Part Two discusses cytoskeletal motors, such as kinesin and myosin; and Part Three talks about nucleic acid motors, such as DNA polymerases, helicases, and nucleosome remodelers. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and comprehensive, Molecular Motors: Methods and Protocols, Second Edition is a valuable resource for (bio)physicistsand molecular/cellular biologists whose research delves into the mechanisms at work in cells and the motors which power them.
Contents
Cellular and Nuclear Forces: An Overview.- The Bacterial Flagellar Rotary Motor in Action.- Purification and Reconstitution of Ilyobacter tartaricus ATP Synthase.- Using Microfluidics Single Filament Assay to Study Formin Control of Actin Assembly.- Engineering Synthetic Myosin Filaments using DNA Nanotubes.- Direct Imaging of Walking Myosin V by High-Speed Atomic Force Microscopy.- High-Resolution Single-Molecule Kinesin Assays at kHz Frame Rates.- Multicolor Tracking of Molecular Motors at Nanometer Resolution.- High-Speed Optical Tweezers for the Study of Single Molecular Motors.- Determining Stable Single Alpha Helical (SAH) Domain Properties by Circular Dichroism and Atomic Force Microscopy.- The Role of Supercoiling in the Motor Activity of RNA Polymerases.- Single-Molecule FRET Analysis of Replicative Helicases.- Recombinases and Related Proteins in the Context of Homologous Recombination Analyzed by Molecular Microscopy.- DNA Organization and Segregation Super-Resolved.- Electrophoretic Analysis of the DNA Supercoiling Activity of DNA Gyrase.- Single Molecule Angular Optical Trapping for Studying Transcription under Torsion.- Anisotropy-Based Nucleosome Repositioning Assay.- Remodeling and Repositioning of Nucleosomes in Nucleosomal Arrays.- Measuring Unzipping and Rezipping of Single Long DNA Molecules with Optical Tweezers.- Single-Molecule Measurements of Motor-Driven Viral DNA Packaging in Bacteriophages Phi29, lambda, and T4 with Optical Tweezers.- Methods for Single Molecule Sensing and Detection Using Bacteriophage Phi29 DNA Packaging Motor.
