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Full Description
This volume describes cell culture protocols for the maintenance, propagation, manipulation, and analysis of primary explanted cells from various mouse organ systems. After introductory chapters that examine the applicability of mouse models as a discovery tool and describe critical factors and variables that influence cell culture endpoints, protocol chapters explore cell culture methodologies for specific mouse cell types and lineages. Written in the highly successful Methods in Molecular Biology series format, these chapters include introductions to their respective topics, comprehensive lists of the essential materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Mouse Cell Culture: Methods and Protocols aims to ensure successful results utilizing these protocols in further studies, leading to the identification of key factors, genes, and cellular pathways regulating cell lineage commitment and differentiation, modeling disease, and identifying novel therapeutic targets.
Contents
The Applicability of Mouse Models to the Study of Human Disease.- Optimizing the Cell Culture Microenvironment.- Propagation and Maintenance of Mouse Embryonic Stem Cells.- Production of High Titer Lentiviral Particles for Stable Genetic Modification of Mammalian Cells.- Generation of Mouse Induced Pluripotent Stem Cells by Lentiviral Transduction.- Gene Editing of Mouse Embryonic and Epiblast Stem Cells.- Identification of Circulating Endothelial Colony Forming Cells from Murine Embryonic Peripheral Blood.- Imaging and Analysis of Mouse Embryonic Whole Lung, Isolated Tissue, and Lineage-Labeled Cell Culture.- Mouse Hematopoietic Stem Cell Modification and Labeling by Transduction and Tracking Post Transplantation.- Genetic Manipulation and Selection of Mouse Mesenchymal Stem Cells for Delivery of Therapeutic Factors In Vivo.- Isolation and Culture of Primary Mouse Middle Ear Epithelial Cells.- Isolation and Propagation of Lacrimal Gland Putative Epithelial Progenitor Cells.- Organotypic Culture of Adult Mouse Retina.- Langendorff-Free Isolation and Propagation of Adult Mouse Cardiomyocytes.- Isolation, Culture, and Characterization of Primary Mouse Epidermal Keratinocytes.- Isolation and Propagation of Mammary Epithelial Stem and Progenitor Cells.- An Organoid Assay for Long-Term Maintenance and Propagation of Mouse Prostate Luminal Epithelial Progenitors and Cancer Cells.- Isolation, Purification, and Culture of Mouse Pancreatic Islets of Langerhans.- Identification and In Vitro Expansion of Adult Hepatocyte Progenitors from Chronically Injured Livers.- The Preparation of Decellularized Mouse Lung Matrix Scaffolds for Analysis of Lung Regenerative Cell Potential.- Mouse Lung Tissue Slice Culture.
