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Full Description
This book describes current methods for the identification and characterization of the major hallmarks of senescent cells. Chapters focus on the high heterogeneity of the senescence phenotypes, and techniques to induce and identify specific senescence programs. Additional chapters describe cellular and mouse models in which is possible to study the complex cell and non-cell autonomous functions of senescent cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Cellular Senescence: Methods and Protocols aims to ensure successful results in the further study of this vital field.
Contents
Detecting Cellular Senescence in Reprogramming.- DNA Damage In situ Ligation followed by Proximity Ligation Assay (DI-PLA).- Reactive Oxygen Species Detection in Senescent Cells.- Cellular Identification and Quantification of Senescence-associated β-Galactosidase Activity in vivo.- Relative Human Telomere Length Quantification by Real-time PCR.- Assessing Functional Roles of the Senescence-associated Secretory Phenotype (SASP).- Measuring the Inflammasome in Oncogene-induced Senescence.- Alarmin Detection in Senescent Cells.- IMR90 ER:RAS: A Cell Model of Oncogene-induced Senescence.- Genotoxic Stress-induced Senescence.- A Multi-parametric Assay to Evaluate Senescent Cells.- A Novel Quantitative Method for the Detection of Lipofuscin, the Main Byproduct of Cellular Senescence, in Fluids.- Measurement of Metabolite Changes in Senescent Cells by Mass Spectrometry.- Quantification of Autophagy during Senescence.- Quantification of Aneuploidy in Mammalian Systems.- Identification of Genomic Alterations Through Multi-Level DNA Structural Analysis.- Mouse Models of Accelerated Cellular Senescence.- Methods to Quantify the NF-kB Pathway during Senescence.
