Full Description
This volume looks at the role of natural antibodies in pathogen elimination, cell survival, inflammation, cancer, and autoimmunity. The chapters in this book cover numerous topics, such as isolation of natural antibodies; methods for separating natural antibodies from human plasma, saliva, breast milk, and gastrointestinal fluids; functional properties of natural antibodies such as anti-tumor cytotoxic activity, and hydrolysis and dissolution of their target antigens; their utility in serological diagnosis of microbial antigens; and the role of natural antibodies in inhibiting viral vectors in the absence of prior exposure to the virus. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Practical and thorough, Natural Antibodies: Methods and Protocols is a valuable resource for researchers and experts who are interested in further studies of natural antibodies.
Contents
Natural Antibodies: Next Steps towards Translational Investigation.- Isolation of Natural Anti-FceRIa Autoantibodies from Healthy Donors.- Isolation of Antibodies from Human Plasma, Saliva, Breast Milk, and Gastrointestinal Fluid.- Purification of Natural Antibodies against Tau Protein by Affinity Chromatography.- Unbiased RACE-Based Massive Parallel Surveys of Human IgA Antibody Repertoires.- Analysis of Signaling Events in B-1a Cells.- Exploring the Role of Microbiota in the Limiting of B1 and MZ B-Cell Numbers by Naturally-Secreted Immunoglobulins.- Assessment of Anti-Tumor Cytotoxic Activity of Naturally Occurring Antibodies in Human Serum or Plasma.- Hydrolysis and Dissolution of Amyloids by Catabodies.- Methods for Post-Translational Induction of Polyreactivity of Antibodies.- Characterization of Natural IgM Antibodies Recognizing Oxidation-Specific Epitopes on Circulating Microvesicles.- Natural Monoclonal Antibody to Oxidized Low-Density Lipoprotein and Aggregatibacter actinomycetemcomitans.- Detection of Natural Antibodies and Serological Diagnosis of Pneumococcal Pneumonia using a Bead-Based High-Throughput Assay.- Detection of Naturally Occurring Human Antibodies against Gangliosides by ELISA.- Evaluating the Impact of Natural IgM on Adenovirus Type 5 Gene Therapy Vectors.
