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Full Description
This is volume presents protocols relevant for plant receptor kinases(PRK) both in development and defence. Chapters guide readers through comprehensive experimental approach for molecular investigations of plant receptor kinases, from the simplest methods for expression and purification of receptor domains to the most advanced methods aiming at understanding the dynamics of receptor complex formation and specificity of signaling pathways. Written in the highly successful Methods
in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Plant Receptor Kinases: Methods and Protocols aims to ensure successful results in the further study of this vital field.
Contents
Expression of Plant Receptor Kinases in E. coli.- Expression of Plant Receptor Kinases in Yeast.- Expression of Plant Receptor Kinases in Tobacco BY-2 Cells.- Cell-free Synthesis of Plant Receptor Kinases.- Purification of Plant Receptor Kinases from Plant Plasma Membranes.- Photoaffinity Labeling of Plant Receptor Kinases.- Quantitative Detection of Oxidative Burst upon Activation of Plant Receptor Kinases.- Extraction and Curation of Gene Models for Plant Receptor Kinases for Phylogenetic Analysis.- In silico Prediction of Ligand Binding Sites of Plant Receptor Kinases using Conservation Mapping.- Co-immunoprecipitation of Plant Receptor Kinases.- Autophosphorylation Assays using Plant Receptor Kinases Synthesized in Cell-free Systems.- Investigation of Autophosphorylation Sites of Plant Receptor Kinases and Phosphorylation of Interacting Partners.- In vitro Assessment of Guanylyl Cyclase Activity ofPlant Receptor Kinases.- BiFC Assay to Detect Calmodulin Binding to Plant Receptor Kinases.- Pull Down Assay to Characterize Ca2+/Calmodulin Binding to Plant Receptor Kinases.- The use of FRET/FLIM to Study Proteins Interacting with Plant Receptor Kinases.- Detection and Analyses of Endocytosis of Plant Receptor Kinases.
