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Full Description
This volume focuses and describes tools, assays, and techniques used to enhance the understanding of the role of auxins and cytokinins. The chapters in this book cover topics such as: microbial manipulation of auxin and cytokinins in plants; interplay between auxin and cytokinin and its impact on MAPK; H2O2 production in Arabidopsis leaves; crosstalk between jasmonate and auxin in plant stress responses of roots; and high-throughput protoplast trans-activation (PTA) screening. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and comprehensive, Auxins and Cytokinins in Plant Biology: Methods and Protocols is a valuable resource to plant scientists, graduate and under graduate students in addressing their biological questions relevant to the functional implications of auxin and cytokinins.
Contents
Methodological Advances in Auxin and Cytokinin Biology.- Analytical Determination of Auxins and Cytokinins.- Manipulation of Auxin and Cytokinin Balance During the Plasmodiophora brassicae: Arabidopsis thaliana Interaction.- Microbial Manipulation of Auxin and Cytokinins in Plants.- A Standardized Method to Assess Infection Rates of Root-Knot and Cyst Nematodes in Arabidopsis thaliana Mutants with Alterations in Root Development Related to Auxin and Cytokinin Signaling.- Reconstruction of an Immune Dynamic Model to Simulate the Contrasting Role of Auxin and Cytokinin in Plant Immunity.- Interplay between Auxin and Cytokinin and its Impact on Mitogen Activated Protein Kinase (MAPK).- Quantification of Cytokinin Levels and Responses in Abiotic Stresses.- Assessment of Cytokinin-Induced Immunity through Quantification of Hyaloperonospora arabidopsidisInfection in Arabidopsis thaliana.- Real-Time Genetic Manipulations of the Cytokinin Pathway: A Tool for Laboratory and Field Studies.- Modulating the Levels of Plant Hormone Cytokinins at the Host-Pathogen Interface.- Analyzing Cytokinin Responses during Plant-Nematode Interactions.- Examining H2O2 Production in Arabidopsis Leaves Upon Challenge by Cytokinin.- A Systems Biology Methodology Combining Transcriptome and Interactome Datasets to Assess the Implications of Cytokinin Signaling for Plant Immune Networks.- Monitoring of Crosstalk between Jasmonate and Auxin in the Framework of Plant Stress Responses of Roots.- High-Throughput Protoplast Trans-Activation (PTA) Screening to Define Transcription Factors in Auxin-Mediated Gene Regulation.
