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Full Description
This volume illustrates how microfluidic approaches can meet the requirement of clinical diagnosis based on molecular or cellular biomarkers. Microchip Diagnostics: Methods and Protocols is divided into four sections describing the business aspects of the microfluidic-based systems for diagnosis, demonstrating how versatile microfluidics can be regarding to protein bioassay integration, presenting microfluidic approaches for nucleic analysis based on mono or diphasic format, and highlighting recent contributions. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Contents
Microfluidics-enabled Diagnostic Systems: Markets, Challenges, and Examples.- Capillary-Driven Microfluidic Chips for Miniaturized Immunoassays: Efficient Fabrication and Sealing of Chips Using a "Chip-olate" Process.- Capillary-driven Microfluidic Chips for Miniaturized Immunoassays: Patterning Capture Antibodies using Microcontact Printing and Dry-film Resists.- Fabrication and Evaluation of Microfluidic Immunoassay Devices with Antibody-immobilized Microbeads Retained in Porous Hydrogel Micropillars.- Using Electrophoretic Immunoassay to Monitor Hormone Secretion.- On-chip Magnetic Particle-based Immunoassays using Multilaminar Flow for Clinical Diagnostic.- Digital Microfluidics Assisted Sealing of Individual Magnetic Particles in fFmtoliter-sized Reaction wells for Single-molecule Detection.- Microfluidic DNA Stretching Device for Single-Molecule Diagnostics.- Droplet Microfluidic and Magnetic Particles Platform for Cancer Typing.- SlipChip Device for Digital Nucleic Acid Amplification.- Multiplex Detection of KRAS Mutations using Passive Droplet Fusion.- Droplet-based Microfluidics Digital PCR for the Detection of KRAS Mutations.- Negative Enrichment of Circulating Tumor Cells in Blood Using a Microfluidic Chip.- Microfluidic Based Bacteria Isolation from Whole Blood for Diagnostics of Blood Stream Infection.- Microfluidic POlatform for Parallel Single Cell Analysis for Diagnostic Applications.- FISH-in-CHIPS.
