Full Description
This volume provides readers with detailed protocols covering the main cancer cytogenetics techniques needed for clinical utilization and research purposes. The chapters in this book cover topics such as chromosome preparation for myeloid malignancies; chromosome bandings; fluorescence in situ hybridization probe preparation; array-based comparative genomic hybridization; and cytogenetic nomenclature and reporting. The updated reviews on chromosomal abnormalities in hematological malignancies are excellent guides for cytogenetics data interpretations and specific malignant diseases correlation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and comprehensive, Cancer Cytogenetics: Methods and Protocolsis a valuable resource for the novice in cytogenetics because it provides helpful guiding protocols, but it's also great for those who are already engaged in the field and are looking for some technical hints.
Contents
Cancer Cytogenetics: An Introduction.- Chromosome Preparation for Myeloid Malignancies.- Chromosome Preparation for Acute Lymphoblastic Leukemia.- Chromosome Preparation for Chronic Lymphoid Malignancies.- Cytogenetic Harvesting of Cancer Cells and Cell Lines.- Chromosome Bandings.- Chromosome Recognition.- Application of Fluorescence In Situ Hybridization Technology in Malignancies.- Fluorescence In Situ Hybridization Probe Preparation.- Fluorescence In Situ Hybridization Probe Validation for Clinical Use.- Fluorescence In Situ Hybridization on Tissue Sections.- Cytoplasmic Immunoglobulin Light Chain Revelation and Interphase Fluorescence In Situ Hybridization in Myeloma.- Quantitate Fluorescence In Situ Hybridization (QFISH).- High Resolution of Fiber-Fluorescence In Situ Hybridization (Fiber-FISH).- Array-Based Comparative Genomic Hybridization (aCGH).- Multicolor Karyotyping and Fluorescence In Situ Hybridization-Banding (MCB/mBAND).- Cytogenetics for Biological Dosimetry.- Recurrent Cytogenetic Abnormalities in Myelodysplastic Syndromes.- Recurrent Cytogenetic Abnormalities in Acute Myeloid Leukemia.- Recurrent Cytogenetic Abnormalities in Myeloproliferative Neoplasms and Chronic Myeloid Leukemia.- Recurrent Cytogenetic Abnormalities in Acute Lymphoid Leukemia.- Recurrent Cytogenetic Abnormalities in Non-Hodgkin's Lymphoma and Chronic Lymphocytic Leukemia.- Recurrent Cytogenetic Abnormalities in Multiple Myeloma.- Cytogenetic Nomenclature and Reporting.- Cytogenetic Resources and Information.
