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Full Description
This volume provides an up-to-date compilation
of current methodological approaches utilized for the exploration of nucleolar
structure and function. Chapters cover
a diversity of protocols that include imaging of the nucleolus, analysis of
ribosomal RNA transcription and processing, and genomics and proteomics of the
nucleolus. Written in the highly successful Methods in Molecular Biology series
format, chapters include introductions to their respective topics, lists of the
necessary materials and reagents, step-by-step, readily reproducible laboratory
protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, The Nucleolus: Methods and Protocols provides scientists with a reliable practical
handbook to facilitate the investigation of this nuclear compartment at the
advanced level.
Contents
The Relationship between Human Nucleolar
Organizer Regions and Nucleoli, Probed by 3D-Immunofish.- Correlative Light and
Electron Microscopy of Nucleolar Transcription in Saccharomyces Cerevisiae.- High-Throughput Live-Cell Microscopy
Analysis of Chromosome Domain - Nucleolus Associations in S. Cerevisiae.- Quantitative Immunofluorescence Analysis of
Nucleolus-Associated Chromatin.- Visualization of The Nucleolus in Living Cells
with Cell-Penetrating Fluorescent Peptides.- Purification of S. Cerevisiae RNA Polymerase I for
Structural and Functional Studies.- Analysis of S. Cerevisiae RNA Polymerase I Transcription In Vitro.- Preparation of Chromatin Templates to Study RNA Polymerase
I Transcription In Vitro.- Measurement
of Rrna Synthesis and Processing by 4su-Tagging.- Metabolic Labeling in the Study
of Mammalian Ribosomal RNA Synthesis.- Quantitative Northern Blot Analysis of
Mammalian Rrna Processing.- Complete
Sequence Construction of the Highly Repetitive Ribosomal RNA Gene Repeats in
Eukaryotes using Whole Genome Sequence Data.- Analysis of Rdna Methylation in Arabidopsis By 2D Gel Electrophoresis.- Fluorescence-Activated
Sorting of Arabidopsis Nucleoli.- Purification
of RNA Polymerase I-Associated Chromatin from Yeast Cells.- Bioinformatic Analysis
of Chip-Seq Data on the Repetitive Rrna Gene.- Deep Sequencing Analysis of Nucleolar
Small Rnas: Isolation and Library Preparation.- Deep Sequencing Analysis of
Nucleolar Small RNAs: Bioinformatics.- Quantitative Proteomic Analysis of the
Human Nucleolus.- Assessing Nucleolar Localization of Proteins: Bioinformatic
Analysis of Proteomics Data.
