Full Description
This volume provides readers with a wide collection of the latest and readily reproducible technical protocols available in the field of
non-viral gene delivery vectors. The chapters in this book are organized into
three major parts: Part I is a section on conventional bolus gene delivery
vectors that introduces typical transfection approaches relying
on the addition of transfectants to the cell culture medium where the cells are
grown in; Part II covers stimuli-responsive bolus transfectants and topics on gene delivery complexes made of smart polymers or
stimuli-responsive polymers that change according to the environment they are
in and delivered by dripping into cells; Part III discusses examples of
substrate-mediated gene delivery—also termed reverse
transfection—and the immobilization of a gene delivery vector onto a surface as
opposed to more typical bolus delivery from the medium. Written in the highly
successful Methods in Molecular Biology series format, chapters include
introductions to their respective topics, lists of the necessary materials and
reagents, step-by-step, readily reproducible laboratory protocols, and tips on
troubleshooting and avoiding known pitfalls.
Cutting-edge and practical, Non-Viral Gene Delivery Vectors: Methods and
Protocols is written for experimentalists, and is an essential part of many
laboratory bookshelves. This book will help novice and professionals alike
succeed in their research in this field.
Contents
Physical Chemical and Biomolecular
Methods for the Optimization of Cationic Lipid-Based Lipoplexes In Vitro
for the Gene Therapy Applications.- Cationic Lipid-Based Nucleic Acid Vectors.- Lipoplexes from Non-Viral Cationic Vectors: DOTAP-DOPE
Liposomes and Gemini Micelles.- Anionic/Zwitterionic Lipid-Based Gene Vectors of pDNA.- Elaboration and Physicochemical Characterization of Niosome-Based
Nioplexes for Gene Delivery Purposes.- Quantitative Intracellular Localization of Cationic Lipid-Nucleic
Acid Nanoparticles with Fluorescence Microscopy.- Targeted
Delivery of Peptide-Tagged DNA Lipoplexes to Hepatocellular Carcinoma Cells.- Lipoplexes Strengthened by Anionic
Polymers: Easy Preparation of Highly Effective siRNA Vectors Based on Cationic Lipids
and Anionic Polymers.- Polymer Based Gene Silencing: In Vitro Delivery of SiRNA.- Polyallylamine
Derivatives: Novel Non-Toxic Transfection Agents.- Biodegradable
Three-Layered Micelles and Injectable Hydrogels.- Cationic Lipid-Coated
Polyplexes ("Lipopolyplexes") for DNA and Small RNA Delivery.- Preparation of Targeted Anionic
Lipid-Coated Polyplexes for MicroRNA Delivery.- Characterization
and Investigation of Redox-Sensitive Liposomes for Gene Delivery.- From Artificial
Amino Acids to Sequence-Defined Targeted Oligoaminoamides.- Gene Delivery
Method Using Photo-Responsive Poly(β-Amino Ester) as Vectors.- Thermo-Responsive
Polyplex Micelles with PEG Shells and PNIPAM Layer to Protect DNA Cores for Systemic
Gene Therapy.- Application of Polyethylenimine-Grafted Silicon Nanowire
Arrays for Gene Transfection.
