Full Description
This volume details a valuable collection of protocols and
reviews, such as emerging experimental and theoretical approaches. These
approaches have resulted in a substantial improvement in the understanding of
chromosome architecture. Chromosome Architecture:
Methods and Protocols guides readers through cutting-edge interdisciplinary
methods which allow for an understanding of architecture of chromosomes with
exceptionally enhanced resolution, both in terms of space and time. Written in the highly successful Methods in
Molecular Biology series format, chapters include introductions to their
respective topics, lists of the necessary materials and reagents, step-by-step,
readily reproducible laboratory protocols, and tips on troubleshooting and
avoiding known pitfalls.
Authoritative
and cutting-edge, Chromosome
Architecture: Methods and Protocols aims to ensure successful results in the
further study of this vital field.
Contents
New Advances in Chromosome Architecture.- Single Molecule
Narrowfield Microscopy Of Protein-DNA Binding Dynamics In Glucose Signal
Transduction Of Live Yeast Cells.- Single-Molecule Imaging to Characterise the Transport
Mechanism of the Nuclear Pore Complex.- Using Fluorescence Recovery after Photobleaching (FRAP)
To Study Dynamics Of The Structural Maintenance Of Chromosome (SMC) Complex In
Vivo.- Imaging DNA Structure by Atomic Force Microscopy.- Investigating Bacterial Chromosome
Architecture Using Epifluorescence Microscopy.- Transverse Magnetic Tweezers
Allowing Coincident Epi-Fluorescence Microscopy On Horizontally Extended DNA.- Probing Chromosome Dynamics in Bacillus Subtilis.- Systems Biology Approaches for Understanding Genome Architecture.- In
Vivo And In Situ Replication Labeling Methods For Super-Resolution
Structured Illumination Microscopy Of Chromosome Territories And Chromatin
Domains.- DNA-Protein Interactions
Studied Directly Using Single Molecule Fluorescence Imaging Of Quantum Dot
Tagged Proteins Moving On DNA Tightropes.- Escherichia Coli Chromosome Copy
Number Measurement Using Flow Cytometry Analysis.- Bacterial Chromosome Dynamics by Locus Tracking In Fluorescence
Microscopy.- Biophysical Characterization of Chromatin Remodelling Protein CHD4.- Atomistic Molecular
Dynamics Simulations of DNA Minicircle Topoisomers: A Practical Guide to Setup,
Performance and Analysis.- Super-Resolution Microscopy and Tracking of DNA-Binding Proteins in Bacterial Cells.- Studying the Dynamics of Chromatin Binding Proteins in Mammalian Cells
Using Single-Molecule Localization Microscopy.- Intra
Nuclear Single Particle Tracking (I-SPT) To Reveal the Functional Architecture Of
Chromosomes.- Visualizing Bacillus Subtilis during Vegetative Growth and Spore Formation.
