Full Description
This volume provides a collection of methods and protocols detailing extracellular RNA and hopes to be a useful resource for those interested in this exciting and emerging field. Chapters are divided into four sections covering an overview of the field, approaches for the isolation of exRNA, protocols for detection and quantitation of exRNA, and approaches that can be useful for studies to evaluate potential therapeutic applications. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Extracellular RNA: Methods and Protocols aims to ensure successful results in the further study of this vital field.
Contents
Extracellular RNAs - A New Awareness of Old Perspectives.- Overview of Protocols for Studying Extracellular RNA and Extracellular Vesicles.- Extracellular RNA Isolation from Cell Culture Supernatant.- Use of a Hollow Fiber Bioreactor to Collect Extracellular Vesicles from Cells in Culture.- Isolation of Extracellular RNA from Serum/Plasma.- Isolation of Extracellular RNA from Bile.- Cushioned-Density Gradient Ultracentrifugation (C-DGUC): a Refined and High Performance Method for the Isolation, Characterization & Use of Exosomes.- Magnetic Particle-Based Immunoprecipitation of Nanoscale Extracellular Vesicles from Biofluids.- Enrichment of Extracellular Vesicle Subpopulations via Affinity Chromatography.- Detection and Analysis of Non-vesicular Extracellular RNA.- Isolation of Plasma Lipoproteins as a Source of Extracellular RNA.- Droplet Digital PCR for Quantitation of Extracellular RNA.- Preparation of Small RNA NGS Libraries from Biofluids.- Multiplexed Detection and Quantitationof Extracellular Vesicle RNA Expression using NanoString.- Milk-derived Extracellular Vesicles for Therapeutic Delivery of Small Interfering RNAs.- Loading of Extracellular Vesicles with Hydrophobically Modified siRNAs.
