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Full Description
This volume provides established approaches for identifying, characterizing, and manipulating circRNAs in vitro, in vivo, and in silico. Chapters highlight the breakthroughs and the challenges in this new field of research. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Circular RNAs: Methods and Protocols aims to useful and informative for further study into this vital field.
Contents
Detection and Reconstruction of Circular RNAs from Transcriptomic Data.- Deep Computational Circular RNA Analytics from RNA-seq Data.- Genome-wide circRNA Profiling from RNA-seq Data.- Analysis of Circular RNAs using the Web Tool CircInteractome.- Characterization and Validation of Circular RNA and their Host Gene mRNA Expression using PCR.- Detecting Circular RNAs by RNA Fluorescence In Situ Hybridization.- Single Molecule Fluorescence In Situ Hybridization (FISH) of Circular RNA CDR1as.- A Highly-efficient Strategy for Overexpressing circRNAs.- Constructing GFP-based Reporter to study Back-splicing and Translation of Circular RNA.- Northern Blot Analysis of Circular RNAs.- Non-radioactive Northern Blot of circRNAs.- Characterization of Circular RNA Concatemers.- Characterization of Circular RNAs (circRNA) Associated with the Translation Machinery.- Synthesis and Engineering of Circular RNAs.- Preparation of Circular RNA in vitro.- Discovering circRNA-microRNA Interactions from CLIP-Seq Data.- Identification of circRNAs for miRNA Targets by Argonaute2 RNA Immunoprecipitation and Luciferase Screening Assays.
