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Full Description
This volume presents a comprehensive overview of contemporary methods to analyze photorespiration in higher plants. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Photorespiration: Methods and Protocols aims to ensure successful results in the further study of this vital field.
Contents
Estimation of Photorespiratory Fluxes by Gas Exchange.- Measurement of Transcripts Associated with Photorespiration and Related Redox Signaling.- Measurement of Enzyme Activities.- In vitro Alkylation Methods for Assessing the Protein Redox State.- Dimethyl-labeling Based Quantification of the Lysine Acetylome and Proteome of Plants.- In vitro Analysis of Metabolite Transport Proteins.- Quantification of Photorespiratory Intermediates by Mass Spectrometry-based Approaches.- Targeted Isolation and Characterization of T-DNA Mutants Defective in Photorespiration.- Exploiting Natural Variation to Discover Candidate Genes Involved in Photosynthesis-Related Traits.- Metabolic Engineering of Photorespiration.- 13CO2 Labelling and Mass Spectral Analysis of Photorespiration.- Isotopically Nonstationary Metabolic Flux Analysis (INST-MFA) of Photosynthesis and Photorespiration in Plants.- Genome-scale Modeling of Photorespiratory Pathway Manipulation.- KineticModelling of Photorespiration.- Investigating the Role of the Photorespiratory Pathway in Non-photosynthetic Tissues.- Studying the Function of the Phosphorylated Pathway of Serine Biosynthesis in Arabidopsis thaliana.- Light Microscopy, Transmission Electron Microscopy and Immunohistochemistry Protocols for Studying Photorespiration.
