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Full Description
This collection thoroughly explores the dynamic and ever-developing field of hemostasis and thrombosis diagnostics and research. After an introductory section covering the basics and preanalytical issues, the book continues with in-depth sections that explore how to get the best outcomes from routine coagulation and specialized hemostasis assays, thrombophilia-related techniques, investigations into bleeding disorders, as well as performance of global assays of hemostasis, and finally post-analytical issues in hemostasis and thrombosis testing. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and practical, Hemostasis and Thrombosis: Methods and Protocols serves as an ideal resource for researchers and diagnostic laboratories seeking expert guidance and working to identify the best methodologies to pursue hemostasis and thrombosis testing.
Contents
Overview of Hemostasis and Thrombosis and Contribution of Laboratory Testing to Diagnosis and Management of Hemostasis and Thrombosis Disorders.- Preanalytical Issues in Hemostasis and Thrombosis Testing.- The Interface between Immunotransfusion and Hemostasis and Thrombosis Testing.- Optimizing the Verification of Mean Normal Prothrombin Time (MNPT) and International Sensitivity Index (ISI) for Accurate Conversion of Prothrombin Time (PT) to International Normalized Ratio (INR).- Performance of Activated Partial Thromboplastin Time (APTT): Determining Reagent Sensitivity to Factor Deficiencies, Heparin, and Lupus Anticoagulants.- Performance and Interpretation of Mixing Tests in Coagulation.- D-Dimer Testing: Laboratory Aspects and Current Issues.- Determination of Fibrinogen and Thrombin Time (TT).- An Overview of Thrombophilia and Associated Laboratory Testing.- Laboratory Testing for Activated Protein C Resistance (APCR).- Assessment of Hereditary Thrombophilia: Performance of Protein C (PC) Testing.- Assessment of Hereditary Thrombophilia: Performance of Protein S (PS) Testing.- Assessment of Hereditary Thrombophilia: Performance of Antithrombin (AT) Testing.- Lupus Anticoagulant Testing: Diluted Russell Viper Venom Time (dRVVT).- Lupus Anticoagulant Testing: Activated Partial Thromboplastin Time (APTT) and Silica Clotting Time (SCT).- Solid Phase Immunoassay for the Detection of Anticardiolipin Antibodies.- Solid Phase Immunoassay for the Detection of Anti-β2 Glycoprotein I Antibodies.- Measuring Direct Oral Anticoagulants.- Laboratory Testing Protocols for Heparin-Induced Thrombocytopenia (HIT) Testing.- One-Stage Factor VIII Assays.- Chromogenic Factor VIII Assays for Improved Diagnosis of Hemophilia A.- Assessment of Factor XIII.- Detection and Measurement of Factor Inhibitors.- Platelet Function Tests: Preanalytical Variables, Clinical Utility, Advantages and Disadvantages.- Platelet Function Analyzed by Light Transmission Aggregometry.- Whole Blood Platelet Aggregometry.- Assessment of Platelet Function in Whole Blood by Flow Cytometry.- Flow Cytometry Protocols for Assessment of Platelet Function in Whole Blood.- Diagnosis or Exclusion of Von Willebrand Disease Using Laboratory Testing.- Laboratory Testing for Von Willebrand Factor Antigen (VWF:Ag).- Laboratory Testing for Von Willebrand Factor Collagen Binding (VWF:CB).- Laboratory Testing for Von Willebrand Factor Ristocetin Cofactor (VWF:RCo).- Laboratory Testing for Von Willebrand Factor Activity by Glycoprotein Ib Binding Assays (VWF:GPIb).- Laboratory Testing for Von Willebrand Factor Factor VIII Binding (for 2N VWD).- Ristocetin-Induced Platelet Aggregation (RIPA) and RIPA Mixing Studies.- Laboratory Testing for Von Willebrand Factor Multimers.- Thrombin Generation Assays (TGA).- The Overall Haemostatic Potential (OHP) Assay.- Thromboelastography (TEG).- Post-Analytical Issues in Hemostasis and Thrombosis Testing.
