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Full Description
Recently, many ground-breaking steps have been made towards better understanding NO/cGMP/PKG pathways, its components, substrates, and their localization within a given cell. These advances were only possible due to the development of sophisticated new techniques in the field. In Guanylate Cyclase and Cyclic GMP: Methods and Protocols expert researchers in the field seek to provide an overview of novel techniques to identify various elements of the NO/cGMP/PKG pathway and further characterize their function, signaling, localization, and importance on a cellular level and in whole animal models providing a higher patho-/physiological integration and relevance. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Guanylate Cyclase and Cyclic GMP: Methods and Protocols seeks to provide scientist current methods and a useful guide towards the possibility to apply these techniques to their own research.
Contents
NO-cGMP: The Past, the Present and the Future.- cGMP Dependent Protein Kinases (cGK).- Enzyme Assays for cGMP Hydrolysing Phosphodiesterases.- Radioimmunoassay for the Quantification of cGMP Levels in Cells and Tissues.- Hyperspectral Imaging of FRET-based cGMP Probes.- Visualization of cGMP with cGi Biosensors.- Advances and techniques to measure cGMP in intact cardiomyocytes.- Real-time Monitoring the Spatio-temporal Dynamics of Intracellular cGMP in Vascular Smooth Muscle Cells.- Methods for Identification of cGKI Substrates.- Approaches for Monitoring PKG1a Oxidative Activation.- Analysis of cGMP Signaling in Adipocytes.- A Genetic Strategy for the Analysis of Individual Axon Morphologies in cGMP Signalling Mutant Mice.- Receptor Binding Assay for NO-Independent Activators of soluble Guanylate Cyclase.- Direct Intrathecal Drug Delivery in Mice for Detecting In Vivo Effects of cGMP on Pain Processing.- The Geisler Method: Tracing Activity Dependent cGMP Plasticity Changes Upon Double Detection of mRNA and Protein on Brain Slices.- Detection of cGMP in the Degenerating Retina.
