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Full Description
Microfluidic techniques are becoming widely incorporated into medical diagnostic systems due to the inherent advantages of miniaturization. In Microfluidic Diagnostics: Methods in Molecular Biology, researchers in the field detail methods and protocols covering subjects such as microfluidic device fabrication, on-chip sample preparation, diagnostic applications and detection methodologies. The protocols described range from cutting-edge developments to established techniques and basic demonstrations suitable for education and training; from basic fabrication methods to commercializing research. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Microfluidic Diagnostics: Methods in Molecular Biology seeks to aid scientists in the further development and commercialization of microfluidic diagnostic technologies
Contents
Present Technology and Future Trends in Point of Care Microfluidic Diagnostics.-Teaching Microfluidic Diagnostics Using Jell-O® Chips.-Fundamentals of Microfluidics for High School Students with no Prior Knowledge of Fluid Mechanics.-Measuring Microchannel Electroosmotic Mobility and Zeta Potential by the Current Monitoring Method.-Overview of the Microfluidic Diagnostics Commercial Landscape.-Practical Aspects of the Preparation and Filing of Patent Applications.-Introduction to In Vitro Diagnostic Device Regulatory Requirements.-Microfluidic Device Fabrication by Thermoplastic Hot-Embossing.-Introduction to Glass Microstructuring Techniques.-Glass Microstructure Capping and Bonding Techniques.-Rapid Prototyping of PDMS Devices using SU‑8 Lithography.-Microfluidic Interface Technology based on Stereolithography for Glass-based Lab-on-a-Chips.-Three-Dimensional, Paper-Based Microfluidic Devices Containing Internal Timers for Running Time-Based Diagnostic Assays.-Thread Based Devices for Low-Cost Diagnostics.-Droplet Based Microfluidics.-Droplet-Based Microfluidics for Binding Assays and Kinetics Based on FRET.-Surface Treatments for Microfluidic Biocompatibility.-Superhydrophobicity for Anti-Fouling Microfluidic Surfaces.-The Application of Microfluidic Devices for Viral Diagnosis in Developing Countries.-Applications of Microfluidics for Molecular Diagnostics.-Quantitative Heterogeneous Immunoassays in Protein Modified Polydimethylsiloxane Microfluidic Channels for Rapid Detection of Disease Biomarkers.-Breast Cancer Diagnostics using Microfluidic Multiplexed Immunohistochemistry.-Charged-coupled Device (CCD) Detectors for Lab-on-a chip (LOC) Optical Analysis.-Multilayer Microfluidic Poly(ethylene glycol)Diacrylate Hydrogels.-Purification of DNA/RNA in a Microfluidic Device.-Agarose Droplet Microfluidics for Highly Parallel and Efficient Single Molecule Emulsion PCR.-Integrated Fluidic Circuits (IFCs) for Digital PCR.-microFIND® Approach to Fluorescent in Situ Hybridization (FISH).-An ELISA Lab-on-a-Chip (ELISA-LOC).-Multiplexed Surface Plasmon Resonance Imaging for Protein Biomarker Analysis.-Surface Acoustic Wave (SAW) Biosensors: Coupling of Sensing Layers and Measurement.-Microchip UV Absorbance Detection Applied to Isoelectric Focusing of Proteins.
