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Full Description
Although embryonic stem cells currently enjoy the public limelight and show great pr- ise for cell based medical therapies, it is the adult stem cells which are responsible for the body's natural ability to fght disease, heal and recover, or fail and succumb to various maladies. The study of mammalian adult stem cells has surged recently, most likely from a maturation of stem cell studies in the classical developmental model organisms and in hematopoeisis. All the tissues of the body examined so far are generated and regenerated from stem cells, it has been an important frst step to adapt or devise new methods to identify and obtain these cells in quantity and purity for further study. Culture techniques have been optimized for managing the growth and differentiation of stem cells in vitro; as some stem cells are pluripotent, often the method is to guide the fate of such cells among the possible differentiation fates. Much of this work, and that in the classical model org- isms, has helped defne the aspects of the stem cell environment or niche that are crucial for both growth and differentiation, and these studies have moved in vivo at increasingly higher resolution. Importantly, the in vivo niche is a current target for bioengineering the matrix and signaling factors. Herein, we present methods for studying six types of mammalian stem cells, m- mary, neural, mesenchymal, endothelial, dendritic, and muscle.
Contents
In Situ Analysis of Cell Populations: Long-Term Label-Retaining Cells.- Limiting-Dilution Transplantation Assays in Mammary Stem Cell Studies.- Use of Stem Cell Markers in Dissociated Mammary Populations.- Isolation of Adult Hippocampal Neural Progenitors.- In Vitro Culture and Analysis of Adult Hippocampal Neural Progenitors.- In Vivo Analysis of Engrafted Adult Hippocampal Neural Progenitors.- Viral Packaging and Transduction of Adult Hippocampal Neural Progenitors.- Culture of Bone Marrow Mesenchymal Stem Cells on Engineered Matrix.- Preparation and Analysis of Endothelial Progenitor Cells Associated with Angiogenesis.- Preparation of Adult Muscle Fiber-Associated Stem/Precursor Cells.- Immuno-Analysis and FACS Sorting of Adult Muscle Fiber-Associated Stem/Precursor Cells.- Altered Expression of NFkB in Ex Vivo Differentiated Dendritic Cells from the Aged Subjects: Implications in Immunotherapy.- Preparation of Collagen-Coated Gels that Maximize In Vitro Myogenesis of Stem Cells by Matching the Lateral Elasticity of In Vivo Muscle.
