Full Description
Since the first edition of Vaccinia Virus and Poxvirology: Methods and Protocols was published, a number of important events related to poxvirology have occurred, such as FDA approval of a culture-based live smallpox vaccine and the vaccination of large numbers of U.S. military and relatively large numbers of U.S. civilians. Novel anti-poxvirus therapeutics have been developed and have been used in emergency settings. The second edition of Vaccinia Virus and Poxvirology expands upon the previous edition with entirely new sets of protocols. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Vaccinia Virus and Poxvirology: Methods and Protocols, Second Edition seeks to aid scientists in continuing to study poxviruses using new tools and approaches.
Contents
Working Safely with Vaccinia Virus: Laboratory Technique and Review of Published Cases of Accidental Laboratory Infections.-In-Fusion® Cloning with Vaccinia Virus DNA Polymerase.-Genetic Manipulation of Poxviruses using Bacterial Artificial Chromosome Recombineering.-Easy and Efficient Protocols for Working with Recombinant Vaccinia Virus MVA.-Isolation of Recombinant MVA using F13L Selection.-Screening for Vaccinia Virus Egress Inhibitors - Separation of IMV, IEV, and EEV.-Imaging of Vaccinia Virus Entry into HeLa Cells.-New Method for the Assessment of Molluscum Contagiosum Virus Infectivity.-An Intradermal Model for Vaccinia Virus Pathogenesis in Mice.-Measurements of Vaccinia Virus Dissemination using Whole Body Imaging: Approaches for Predicting of Lethality in Challenge Models and Testing of Vaccines and Anti-viral Treatments.-Mousepox, a Small Animal Model of Smallpox.-Analyzing CD8 T Cells in Mouse Models of Poxvirus Infection.-Generation and Characterization of Monoclonal Antibodies Specific for Vaccinia Virus.-Bioinformatics for Analysis of Poxvirus Genomes.-Antigen Presentation Assays to Investigate Uncharacterized Immunoregulatory Genes.-Characterization of Poxvirus Encoded Proteins that Regulate Innate Immune Signaling Pathways.-Application of Quartz Crystal Microbalance with Dissipation Monitoring (QCM-D) Technology for Studying Interactions of Poxviral Proteins with their Ligands.-Central Nervous System Distribution of the Poxviral Proteins After Intranasal Administration of Proteins and Titering of Vaccinia Virus in the Brain after Intracranial Administration.
