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Full Description
Since the publication of the popular first edition, genomic methods have become more accessible, allowing antibiotic researchers to probe not only the sequence of antibiotic resistance determinants but the mechanism whereby they are expressed and regulated. That, in concert with array technology and an understanding of the importance of biofilms, has greatly expanded antibiotic resistance knowledge. In order to reflect the growing field, Antibiotic Resistance Protocols, Second Edition fully updates and builds upon its first edition with contributions from leading researchers. Beginning with chapters on epidemiology and population genetics, the book continues with sections covering genomics and gene expressions, fitness mutation and physiology, and the detection of resistance. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and up-to-date, Antibiotic Resistance Protocols, Second Edition brings together examples of a diverse range of modern techniques applied in antibiotic research in order to best aid scientists in planning their own future research.
Contents
Epidemiology and Population Genetics.- The RM Test for Determining Methicillin-Resistant Staphylococcus aureus Lineages.- Unravelling the Ecology of Antibiotic Resistant Bacteria in the Nasopharynx.- Genomics and Gene Expression.- Methods to Determine Antibiotic Resistance Gene Silencing.- Studying Gene Induction of Glycopeptide Resistance Using Gene Swapping.- Real-Time PCR Methods to Study Expression of Genes Related to Hypermutability.- Use of DNA Arrays to Study Transcriptional Responses to Antimycobacterial Compounds.- Fitness Mutation and Physiology.- Assays of Sensitivity of Antibiotic-Resistant Bacteria to Hydrogen Peroxide and Measurement of Catalase Activity.- Measurements of Heme Levels and Respiration Rate in Salmonella enterica Serovar Typhimurium.- Methods to Determine Fitness in Bacteria.- Application of Continuous Culture for Measuring the Effect of Environmental Stress on Mutation Frequency in Mycobacterium tuberculosis.- Detection of Resistance.- Identification of Efflux-Mediated Multi-drug Resistance in Bacterial Clinical Isolates by Two Simple Methods.- Evaluation of Efflux Activity of Bacteria by a Semi-automated Fluorometric System.- Measuring the Activity of Active Efflux in Gram-Negative Bacteria.- Rapid Characterization of ?-Lactamases by Multiplex PCR.- Rapid Methods for Testing Inhibitors of Mycobacterial Growth.- Use of Biofilm Model Systems to Study Antimicrobial Susceptibility.- Rapid Analysis of Resistant Mutant Genotypes Using Pyrosequencing®.
