Full Description
Bioluminescent Imaging: Methods and Protocols distills a wide range of techniques that use bioluminescence imaging as a tool for visualizing and tracking various biological processes. Covering diverse fields such as cellular and molecular biology, oncology, neurology, infectious diseases, immunology, and others, the detailed chapters of this volume are arranged by topic and describe practical procedures and applications of different bioluminescent reporters, from photoproteins (Aequorin) to bacterial luciferases as well as other secreted (such as Gaussia) and non-secreted luciferases (such as Firefly). Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and expert tips for troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Bioluminescent Imaging: Methods and Protocols aims to provide diverse and comprehensive techniques to researchers interested in implementing bioluminescence-based imaging in their laboratory, regardless of their previous level of experience with such methodologies.
Contents
Bioluminescence Imaging: Basics and Practical Limitations.- Extraction and Quantification of Adenosine Triphosphate in Mammalian Tissues and Cells.- Neuronal Network Imaging in Acute Slices Using Ca2+ Sensitive Bioluminescent Reporter.- Gaussia Luciferase-Based Mycoplasma Detection Assay in Mammalian Cell Culture.- Split Gaussia Luciferase for Imaging Ligand-Receptor Binding.- Video-Rate Bioluminescence Imaging of Protein Secretion from a Living Cell.- Bioluminescence Reporter Gene-Based Detection of MicroRNAs.- Monitoring of Transcriptional Dynamics of HIF and NFκB Activities.- Real-Time Bioluminescent Tracking of Cellular Population Dynamics.- Fabrication of Bioluminescent Capsules and Live-Cell Imaging.- The Bioluminescent Imaging of Spontaneously Occurring Tumors in Immunocompetent ODD-Luciferase Bearing Transgenic Mice.- Blood-Based Assay with Secreted Gaussia Luciferase to Monitor Tumor Metastasis.- Bioluminescence Imaging of Fungal Biofilm Development in Live Animals.- Bioluminescent Imaging of Bacteria During Mouse Infection.- Cell-Based Bioluminescence Screening Assays.- Bioluminescence-Based Monitoring of Virus Vector-Mediated Gene Transfer in Mice.- Simultaneous In Vivo Monitoring of Regulatory and Effector T Lymphocytes Using Secreted Gaussia Luciferase, Firefly Luciferase, and Secreted Alkaline Phosphatase.- Multiplex Functional Bioluminescent Reporters Using Gaussia Luciferase Fused to Epitope Tags in an Immunobinding Assay.- Non-Invasive In Vivo Monitoring of Extracellular Vesicles.- In Vitro and In Vivo Demonstrations of Fluorescence by Unbound Excitation from Luminescence (FUEL).
