Full Description
Although antiviral drugs have been successfully developed for some viral diseases, there remains a clear, unmet medical need to develop novel antiviral agents for the control and management of many viruses that currently have no or limited treatment options as well as a need to overcome the limitations associated with the existing antiviral drugs, such as adverse effects and emergence of drug-resistant mutations. The second edition of Antiviral Methods and Protocols features:
All chapters are new and written by experts in the field, reflecting the major recent technical advances in antiviral research and discovery.
This edition focuses on many important human viruses, such as human immunodeficiency virus type 1 (HIV-1), hepatitis viruses (hepatitis B and C viruses), herpes viruses, human respiratory syncytial virus (RSV), and influenza virus, while also featuring some important emerging viruses, such as dengue virus, West Nile virus, and chikungunya virus.
As a volume in the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and cutting-edge, Antiviral Methods and Protocols, Second Edition will serve as an excellent laboratory reference for pharmaceutical and academic biologists, medicinal chemists, and pharmacologists as well as for virologists in the field of antiviral research and drug discovery.
Contents
A Fluorescence-Based High Throughput Screening Assay to Identify HIV-1 Inhibitors.- A Homogeneous Time-Resolved Fluorescence Assay to Identify Inhibitors of HIV-1 Fusion.- Identification of HIV-1 Reverse Transcriptase Inhibitors Using a Scintillation Proximity Assay.- Biochemical Screening Assays to Identify HIV-1 Integrase Inhibitors.- HIV-1 Genotyping of the Protease-Reverse Transcriptase and Integrase Genes to Detect Mutations that Confer Anti-Retroviral Resistance.- In Vitro Kinetic Profiling of Hepatitis C Virus NS3 Protease Inhibitors by Progress Curve Analysis.- A Novel Hepatitis C Virus NS5B Polymerase Assay of De Novo Initiated RNA Synthesis Directed from a Heteropolymeric RNA Template.- Selecting and Characterizing Drug-Resistant Hepatitis C Virus Replicon.- A Cellular Replicon-Based Phenotyping Assay to Determine Susceptibility of Hepatitis C Virus Clinical Isolates to NS3/4A Protease Inhibitors.- Expression and Purification of Hepatitis C Virus Protease from Clinical Samples.- Surface Plasmon Resonance as a Tool to Select Potent Drug Candidates for Hepatitis C Virus NS5B Polymerase.- Amplification and Sequencing of the Hepatitis C Virus NS3/4A Protease and the NS5B Polymerase Regions for Genotypic Resistance Detection of Clinical Isolates of Subtypes 1a and 1b.- A Southern Blot Assay for Detection of Hepatitis B Virus Covalently Closed Circular DNA from Cell Cultures.- In Vitro Phenotyping of Recombinant Hepatitis B Virus Containing the Polymerase/Reverse Transcriptase Gene from Clinical Isolates.- Cell-Based Antiviral Assays for Screening and Profiling Inhibitors Against Dengue Virus.- A Duplex Real-Time RT-PCR Assay for Profiling Inhibitors of Four Dengue Serotypes.- High Throughput Screening Using Dengue Virus Reporter Genomes.- Fluorimetric and HPLC-Based Dengue Virus Protease Assays Using aFRET Substrate.- Expression and Purification of Dengue Virus NS5 Polymerase and Development of a High Throughput Enzymatic Assay for Screening Inhibitors of Dengue Polymerase.- Detection and Quantification of Flavivirus NS5 Methyl-transferase Activities.- Testing Antiviral Compounds in a Dengue Mouse Model.- Construction of Self-Replicating Subgenomic West Nile Virus Replicons for Screening Antiviral Compounds.- Methods for Screening and Profiling Inhibitors of Herpes Simplex Viruses.- In Vivo Evaluation of Antiviral Efficacy Against Genital Herpes Using Mouse and Guinea Pig Models.- A Fluorescence-Based High Throughput Screening Assay for Identifying Human Cytomegalovirus Inhibitors.- A Fluorescence-Based High Throughput Antiviral Compound Screening Assay Against Respiratory Syncytial Virus.- Screening and Evaluation of Anti-Respiratory Syncytial Virus Compounds in Cultured Cells.- Evaluation of Antiviral Efficacy Against Human Respiratory Syncytial Virus Using Cotton Rat and Mouse Models.- In Vivo Evaluation of Antiviral Compounds on Respiratory Syncytial Virus Using a Juvenile Vervet Monkey (Chlorocebus pygerythrus) Infection Model.- Methods to Determine Mechanism of Action of Anti-Influenza Inhibitors.- Methods for Evaluation of Antiviral Efficacy Against Influenza Virus Infections in Animal Models.- Development of a High Throughput Antiviral Assay for Screening Inhibitors of Chikungunya Virus and Generation of Drug-Resistant Mutations in Cultured Cells.- A Mouse Model of Chikungunya Virus with Utility in Antiviral Studies.
