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Full Description
Meiosis is one of the most critical processes in eukaryotes, required for continuation of species and generation of new variation. In plants, meiotic recombination is by far the most important source of genetic variation. In Plant Meiosis: Methods and Protocols, expert researchers in the field detail methods for molecular cytogenetics and chromosome analysis in plants. These state-of -the-art protocols allow studying the organization and behavior of the genetic material in a wide range of both model and crop species. Written in the highly successful Methods in Molecular Biology™series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Plant Meiosis: Methods and Protocols provides and extensive list of protocols developed and used in a number of laboratories at the cutting edge of meiosis and chromosome research.
Contents
Spreading and Fluorescence in situ Hybridization (FISH) of Male and Female Meiocyte Chromosomes from Arabidopsis thaliana for Cytogenenetical Analysis.- Analysis of Plant Meiotic Chromosomes by Chromosome Painting.- Using Sequential Fluorescence and Genomic in situ Hybridization (FISH and GISH) to Distinguish the A and C Genomes in Brassica napus.- Labeling Meiotic Chromosomes in Maize with Fluorescence in situ Hybridization.- Examining Female Meiocytes of Maize by Confocal Microscopy.- Three-dimensional Acrylamide Fluorescence in situ Hybridization (FISH) for Plant Cells.- Analyzing Maize Meiotic Chromosomes with Super-resolution Structured Illumination Microscopy.- Live Imaging of Chromosome Dynamics.- Immunolocalization of Meiotic Proteins in Brassicacae - Method .- Immunolocalization of Meiotic Proteins in Arabidopsis thaliana - Method 2.- Immunolocalization Protocols for Visualizing Meiotic Proteins in Arabidopsis thaliana - Method 3.- A Time Course for the Analysis of Meiotic Progression in Arabidopsis thaliana.- Analyzing Meiotic Chromosomes in Rice.- Analysing Meiosis in Barley.- Preparing SC Spreads with RNs for EM Analysis.- Analysis of the Synaptonemal Complex in Brassica using TEM.- Preparing Thin Sections of Meiotic Nuclei for Transmission Electron Microscopy.- Characterization of Meiotic Non-crossover Molecules from Arabidopsis thaliana Pollen.- Chromatin Immunoprecipitation for Studying Chromosomal Localization of Meiotic Proteins in Maize.-Analyzing the Meiotic Transcriptome using Isolated Meiocytes of Arabidopsis thaliana.- Analysis of Meiotic Protein Complexes from Arabidopsis and Brassica using Affinity-based Proteomics.- Identifying Meiotic Mutants in Arabidopsis thaliana.
