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Full Description
Genetic approaches to understanding plant growth and development have always benefitted from screens that are simple, quantitative and rapid. Visual screens and morphometric analysis have yielded a plethora of interesting mutants and traits that have provided insight into complex regulatory pathways, and yet many genes within any given plant genome remain undefined. The premise underlying High Throughput Phenotyping in Plants: Methods and Protocols is that the higher the resolution of the phenotype analysis the more likely that new genes and complex interactions will be revealed. The methods described in this volume can be generally classified as quantitative profiling of cellular components, ranging from ions to small molecule metabolites and nuclear DNA, or image capture that ranges in resolution from chlorophyll fluorescence from leaves and time-lapse images of seedling shoots and roots to individual plants within a population at a field site. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible, High Throughput Phenotyping in Plants: Methods and Protocols serves as an invaluable guide to plant researchers and all scientists who wish to better understand plant growth and development.
Contents
Image-Based Analysis of Light-Grown Seedling Hypocotyls in Arabidopsis.- High Throughput Phenotyping of Plant Shoots.- High Throughput Phenotyping of Root Growth Dynamics.- LEAF GUI: Analyzing the Geometry of Veins and Areoles Using Image Segmentation Algorithms.- Remote Chlorophyll Fluorescence Measurements with the Laser Induced Fluorescence Transient (LIFT) Approach.- Leaf Hue Measurements: A High Throughput Screening of Chlorophyll Content.- High Resolution, Time-Lapse Imaging for Ecosystem-Scale Phenotyping in the Field.- High Throughput Phenotyping of Plant Populations Using a Personal Digital Assistant.- High Throughput Fractionation of Natural Products for Drug Discovery.- Conducting Molecular Biomarker Discovery Studies in Plants.- Highly Sensitive High Throughput Profiling of Six Phytohormones Using MS-Probe Modification and Liquid Chromatography-Tandem Mass Spectrometry.- Qualitative and Quantitative Screening of Amino Acids in Plant Tissues.- Arabidopsis thaliana Membrane Lipid Molecular Species and Their Mass Spectral Analysis.- ICP-MS as a Tool For High Throughput Analysis of Plants.- The Plant Volatilome: Methods of Analysis.- High Throughput Monitoring of Plant Nuclear DNA Contents via Flow Cytometry.- Transient RNAi Assay in 96-Well Plate Format Facilitates High Throughput Gene Function Studies in planta.- A High Throughput Biological Conversion Assay for Determining Lignocellulosic Quality.- Carbohydrate Microarrays in PlantScience.
