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Full Description
Recent years have seen significant advancements in the development of enabling technologies that facilitate the study of Transcription Factors (TFs). TFs are pivotal in the regulation of plant development, reproduction, intercellular signaling, response to environment, cell cycle, and metabolism. Plant Transcription Factors: Methods and Protocols offers a comprehensive approach by covering the basic concepts as well as the detailed protocols of a series of commonly used tools for investigating plant TFs. From discussing select TF families in plants to presenting approaches for identifying them, methods are covered to verify the function, to identify protein interactions in which TFs are involved, and how the interactions are mediated. Increasing examples of TFs that function non-cell-autonomously are being discovered and methods to assess intercellular trafficking are also addressed. A section is devoted to examining interaction with DNA, and the volume concludes with a discussion of directed evolution to generate transcription factors that can more efficiently control desired processes. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and accessible, Plant Transcription Factors: Methods and Protocols serves as an ideal guide to seasoned plant molecular biologists as well as scientists new to the field of TFs and provides many necessary methods to all scientists who are interested in exploring the functions of transcription factors.
Contents
MADS & More: Transcription Factors that Shape the Plant.- In Silico Mining and PCR Based Approaches to Transcription Factor Discovery in Non-Model Plants: Gene Discovery of the WRKY Transcription Factors in Conifers.- Isolation of Plant Transcription Factors Using a Yeast One-Hybrid System.- A Transposon Based Activation Tagging System for Gene Function Discovery in Arabidopsis.- CRES-T, An Effective Gene Silencing System Utilizing Chimeric Repressors.- Analysis of a Transcription Factor Using Transient Assay in Arabidopsis Protoplasts.- Microarray-Based Identification of Transcription Factor Target Genes.- Yeast Protein-Protein Interaction Assays and Screens.- Mapping Functional Domains of transcription factors.- Bimolecular fluorescence complementation as a tool to study interactions of regulatory proteins in plant protoplasts.- Isolation of transcription factor complexes from Arabidopsis cell suspension cultures by tandem affinity purification.- Assaying Transcription Factor Stability.- How to assess the intercellular trafficking of transcription factors.- SELEX (Systematic Evolution of Ligands by EXponential Enrichment), as a powerful tool for deciphering the protein-DNA interaction space.- Footprinting and Missing Nucleoside Analysis of Transcription Factor-DNA Complexes.- Chromatin immunoprecipitation to verify or to identify In Vivo protein-DNA interactions.- Visualizing and characterizing In Vivo DNA-Binding Events and Direct Target Genes of Plant Transcription Factors.- Mapping In Vivo Protein-DNA Interactions in Plants by DamID, a DNA Adenine Methylation-Based Method.- Directed Evolution through DNA Shuffling for the Improvement and Understanding of Genes and Promoters
