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Full Description
Recently, important new findings in the polyamine field and a variety of new experimental systems have revolutionized the study of these ubiquitous cellular components, essential for normal growth and development. In Polyamines: Methods and Protocols, leading researchers contribute an extensive collection of up-to-date laboratory techniques for the further pursuit of polyamine study. The volume delves into vital subjects such as neoplasia studies with animal models and human patients, therapeutic roles for polyamine inhibitors and analogs, polyamine metabolism and oxidative damage, polyamines as regulators of critical ion channels, as well as polyamine transport systems and polyamine-responsive genes. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and expert notes on troubleshooting and avoiding known pitfalls.
Comprehensive and cutting-edge, Polyamines: Methods and Protocols provides a key resource for all scientists pursuing the study of this dynamic and significant aspect of cellular biology.
Contents
Current Status of the Polyamine Research Field.- Exploring Polyamine Biosynthetic Diversity through Comparative and Functional Genomics.- Characterization of Genes for Polyamine Modulon.- Posttranscriptional Regulation of Gene Expression in Epithelial Cells by Polyamines.- Identification, Chemical Synthesis, and Biological Functions of Unusual Polyamines Produced by Extreme Thermophiles.- Polyamine Block of Inwardly-Rectifying Potassium Channels.- Carcinogenesis Studies in Mice with Genetically Engineered Alterations in Polyamine Metabolism.- Transgenic Rodents with Altered SSAT Expression as Models of Pancreatitis and Altered Glucose and Lipid Metabolism.- Use of (Gyro) Gy and Spermine Synthase Transgenic Mice to Study Functions of Spermine.- A Simple Assay for Mammalian Spermine Oxidase: A Polyamine Catabolic Enzyme Implicated in Drug Response and Disease.- Characterization, Assay, and Substrate Specificity of Plant Polyamine Oxidases.- Assay of Deoxyhypusine Synthase Activity.- Assay of Deoxyhypusine Hydroxylase Activity.- Identification and Assay of Allosteric Regulators of S-Adenosylmethionine Decarboxylase.- Protocols for Studying Antizyme Expression and Function.- Identification, Assay, and Functional Analysis of the Antizyme Inhibitor Family.- Post-Transcriptional Regulation of Ornithine Decarboxylase.- Identification and Assays of Polyamine Transport Systems in Escherichia coli and Saccharomyces cerevisiae.- Genetic and Biochemical Analysis of Protozoal Polyamine Transporters.- Heparan Sulfate Proteoglycan-Mediated Polyamine Uptake.- Polyamine Transport Systems in Mammalian Cells and Tissues.- Procedures to Evaluate the Importance of Dietary Polyamines.- Determination of N1,N12-Diacetylspermine in Urine: A Novel Tumor Marker.- Spermidine/spermine-N1-Acetyltransferase in Kidney Ischemia Reperfusion Injury.- Use of Polyamine Metabolites as Markers for Stroke and Renal Failure.-Methods to Evaluate Alterations in Polyamine Metabolism Caused by Helicobacter pylori Infection.- High-Resolution Capillary Gas Chromatography in Combination with Mass Spectrometry for Quantification of Three Major Polyamines in Post-Mortem Brain Cortex.- Spermine Synthase Deficiency Resulting in X-Linked Intellectual Disability (Snyder-Robinson Syndrome).- Methylated Polyamines as Research Tools.- Fluorescent Substrates for Polyamine Catabolic Enzymes and Transport.- Use of Polyamine Derivatives as Selective Histone Deacetylase Inhibitors.- Measurement of Polyamine pKa Values.- Polyamine Analysis by LC-MS.
