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Full Description
Despite being known and studied for years, peptides have never before attracted enough attention to necessitate the invention of the term "peptidomics" in order to specify the study of the complement of peptides from a cell, organelle, tissue or organism. In Peptidomics: Methods and Protocols, expert researchers present a comprehensive range of analytical techniques for the analysis of the peptide contents of complex biological samples with an emphasis often on higher throughput techniques, suitable for the analysis of large numbers of peptides typically present in the peptidomes. Encompassing a number of species ranging from bacteria to man, the methods presented intensively cover topics such as organism handling, tissue and organ dissection, cellular and subcellular fractionation, peptide extraction, fractionation and purification, structural characterization, molecular cloning, and sequence analysis. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.
Comprehensive and cutting-edge, Peptidomics: Methods and Protocols brings this ten year old field fully up-to-date in order to inspire novices and experts alike with the easy-to-follow practical advice needed to set up and carry out analysis of the peptide contents of complex biological samples.
Contents
Peptidomics: Divide et Impera.- From Bacteria to Men.- Performing Comparative Peptidomics Analyses of Salmonella from Different Growth Conditions.- Approaches to Identify Endogenous Peptides in the Soil Nematode Caenorhabditis elegans.- Mass Spectrometric Analysis of Molluscan Neuropeptides.- Monitoring Neuropeptides In Vivo via Microdialysis and Mass Spectrometry.- Protocols for Peptidomic Analysis of Spider Venoms.- Purification and Characterization of Biologically ctive Peptides from Spider Venoms.- MALDI-TOF Mass Spectrometry Approaches to the Characterisation of Insect Neuropeptides.- Direct MALDI-TOF Mass Spectrometric Peptide Profiling of Neuroendocrine Tissue of Drosophila.- Direct Peptide Profiling of Brain Tissue by MALDI-TOF Mass Spectrometry.- Peptidomic Analysis of Single Identified Neurons.- Identification and Analysis of Bioactive Peptides in Amphibian Skin Secretions.- An Efficient Protocol for DNA Amplification of Multiple Amphibian Skin Antimicrobial Peptide cDNAs.- Combined Peptidomics and Genomics Approach to the Isolation of Amphibian Antimicrobial Peptides.- Identification and Relative Quantification of Neuropeptides from the Endocrine Tissues.- Peptidome Analysis of Mouse Liver Tissue by Size Exclusion Chromatography Prefractionation.- Rat Brain Neuropeptidomics: Tissue Collection, Protease Inhibition, Neuropeptide Extraction, and Mass Spectrometric Analysis.- Quantitative Neuroproteomics of the Synapse.- Peptidomics Analysis of Lymphoblastoid Cell Lines.- Peptidomics: Identification of Pathogenic and Marker Peptides.- Tools and Approaches.- Peptidomic Approaches to the Identification and Characterization of Functional Peptides in Hydra.- Immunochemical Methods for the Peptidomic Analysis of Tachykinin Peptides and Their Precursors.- Affinity Peptidomics: Peptide Selection and Affinity Capture on Hydrogels and Microarrays.- In Situ Biosynthesis of Peptide Arrays.- Bioinformatic Approaches to the Identification of Novel Neuropeptide Precursors.- Bioinformatic Identification of Plant Peptides.
