Full Description
Transcription Factor Regulatory Methods details various techniques ranging from cutting-edge to general techniques use to study transcription factor regulatory networks. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Transcription Factor Regulatory Methods aids scientists in the further study into post-genomic or the personal genomic era.
Contents
Detecting Protein-protein Interactions/Complex Components using Mass Spectrometry Coupled Techniques.- The Analysis of Transcription Factor Networks using the IVV Method.- Next-generation Sequencing Coupled with a Cell-free Display Technology for Reliable Interactome of Translational Factors.- Chromatin Immunoprecipitation Protocol for Mammalian Cells.- Detecting Protein-DNA Interactions using a Modified Yeast One-hybrid System.- RNA Sequencing: From Sample Preparation to Analysis.- Detecting Expressed Genes using CAGE.- A Tutorial to Identify Nonlinear Associations in Gene Expression Time Series Data.- Inference of TFRNs (2).- Identification of the Minimal Connected Network of Transcription Factors by Transcriptomic and Genomic Data Integration.- Modeling and Simulation using Cell Designer.- Human Genome Network Platform: A Resource for TFRN Analysis.- Identification of Transcription Factors Activated in Thymic Epithelial Cells During Embryonic Thymus Development.- Analysis of NFATc1-centered Transcription Factor Regulatory Networks in Osteoclast Formation.- Transcriptional Regulation in Adipogenesis through PPARγ-dependent and -Independent Mechanisms by Prostaglandins.- Analysis of TFRNs Associated with Steroid Hormone-related Cancers.- Repositioning Monocyte TFRN into Fibroblasts.
