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Full Description
Mast Cells: Methods and Protocols, Second Edition expands upon the previous edition with current, detailed reviews that cover topics of interest to mast cell neophytes and cognoscenti alike. With new chapters on methods for the enumeration of tissue mast cells and isolation of mature mast cells and mast cell progenitors from mammalian tissues, Danio rerio (zebrafish) model in the study of mast cell development and function, the functions of mast cells in human health and disease and methods for the isolation, methods for the investigation of the molecular mechanisms of mast cell activation and their effector functions, assays for the detection and analysis of mast cell secretory and cell surface phenotype and, mast cell activation state, and experimental mouse models of disease that have been deemed useful for the assessment of mast cell functions in the regulation of innate and adaptive immune response in cancer, tissue fibrosis, auto-inflammation and allergicdisease. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Mast Cells: Methods and Protocols, Second Edition provides a sampler of useful methods and techniques in the further study of mast cell biology.
Contents
Paul Ehrich's Mastzellen: A Historical Perspective of Relevant Developments in Mast Cell Biology.- The Phylogenetic Profile of Mast Cells.- Mast Cell Development and Function in the Zebrafish.- Human Mast Cell and Basophil/Eosinophil Progenitors.- Methods for the Study of Mast Cell Recruitment and Accumulation in Different Tissues.- Notch2 Signaling in Mast-cell Development & Distribution in the Intestine.- Mast Cells in Human Health and Disease.- The Emerging Prominence of the Cardiac Mast Cell as a Potent Mediator of Adverse Myocardial Remodeling.- The Parasympathetic Nervous System as a Regulator of Mast Cell Function.- Growth of Human Mast Cells from Bone Marrow and Peripheral Blood-Derived CD34+ Pluripotent Hematopoietic Cells.- Isolation and Characterization of Human Intestinal Mast Cells.- Human Mast Cell Activation with Viruses and Pathogen Products.- Basic Techniques to Study FceRI Signaling in Mast Cells.- Membrane-cytoskeleton Dynamics in the Course of Mast Cell Activation.- FcεRI Expression and Dynamics on Mast Cells.- Regulation of Mast Cell Survival and Apoptosis.- Protein Tyrosine Phosphatases in Mast Cell Signaling.- MicroRNA Function in Mast Cell Biology:Protocols to Characterize and Modulate microRNA Expression.- Assay of Mast Cell Mediators.- Induction Of Mast Cell Apoptosis By A Novel Secretory Granule-Mediated Pathway.- Measurement of Nitric Oxide in Mast Cells with the Fluorescent Indicator DAF-FM Diacetate.- Real-time Imaging of Ca2+ Mobilization and Degranulation in Mast Cells.- Flow Cytometry-based Monitoring of Mast Cell Activation.- Measurement of Mast Cell Surface Molecules using High-throughput Immunophenotyping using Transcription (HIT).- Investigatiotion using Novel Mouse Models of Mast Cell Deficiency and Mast Cell-specific Gene Inactivation.- Evaluation of Synovial Mast Cell Functions in Autoimmune Arthritis.- Methods for the Study of Mast Cells in Cancer.- Studying Mast Cells in Peripheral Tolerance byusing a Skin Transplantation Model.- The Function of Mast Cells in Autoimmune Glomerulonephritis.- A Mouse Model of Atopic Dermatitis.- Mouse Models of Allergic Asthma.- Methods in Assessment of Airway Reactivity in Mice.
