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Full Description
This volume brings together a unique collection of protocols that cover standard, novel, and specialized techniques. Cell Cycle Oscillators: Methods and Protocols guides readers through recent progress in the field from both holistic and reductionist perspectives, providing the latest developments in molecular biology techniques, biochemistry, and computational analysis used for studying oscillatory networks. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Cell Cycle Oscillators: Methods and Protocols will serve as an invaluable reference to gain further insight into the complex and incompletely understood processes that are involved in the cell cycle and its regulation by oscillatory networks.
Contents
Cell Cycle Control: A System of Interlinking Oscillators.- Model Organisms for Studying the Cell Cycle.- Role of Computational Modelling in Understanding Cell Cycle Oscillators.- E2F Transcription Factors Control the Roller Coaster Ride of Cell Cycle Gene Expression.- Cell Synchronization of Mouse Embryonic Fibroblasts.- Cell Cycle Synchronisation in Xenopus Egg Extracts.- Elutriation for Cell Cycle Synchronization in Fission Yeast.- Spatiotemporal Investigation of Phosphorylation Events during Cell Cycle Progression.- Cell Cycle Dynamics of Proteins and Post-translational Modifications Using Quantitative Immunofluorescence.- Building a Synthetic Transcriptional Oscillator.- Time Series Simulations and Bifurcation Analyses of a Model of Mitotic Oscillations in Xenopus Cell-free Extracts.- A Computational Method for Identifying Yeast Cell Cycle Transcription Factors.- Measuring Activity and Specificity of Protein Phosphatases.- Combining the Optimized Yeast Cytosine Deaminase Protein Fragment Complementation Assay and an in vitro Cdk1 Targeting Assay to Study the Regulation of the g-Tubulin Complex.- Cell Cycle Synchronisation Using a Microfluidic Synchroniser for Fission Yeast Cells.- Detection of Protein-Protein Interactions in Tobacco BY-2 cells Using BimolecularFluorescence Complementation.- Tracking the Cyclin B1-GFP Sensor to Profile the Pattern of Mitosis Versus Mitotic Bypass.- Measuring APC/C Dependent Ubiquitylation in vitro.- Using the Fly-FUCCI System for the Live Analysis of Cell Cycle Dynamics in Cultured Drosophila cells.- Imaging Cell Cycle Phases and Transitions of LivingCells From Yeast to Woman.- Measurement of Cdk1/cyclin B Kinase Activity by Specific Antibodies and Western Blotting.- The use of SNAP Labeling to Study Cell Cycle Oscillatory Proteins.
