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Full Description
In Flavins and Flavoproteins: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used to study flavins and flavoproteins. These include review style methods and protocols to exemplify the variety, the power and the success of modern techniques and methods in application to flavoproteins. Part I of this Volume covers general properties, syntheses and applications of free flavins as well as its analogs and flavoproteins. Part II covers characterizations of flavins and flavoproteins using modern experimental techniques as well as theoretical methods. Written in the highly successful Methods in Molecular Biology series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory.
Thorough and intuitive, Flavins and Flavoproteins: Methods and Protocols aids scientists in continuing to tackle the countless questions that need to be answered to more fully comprehend the vast diversity and specificity of flavin-governed biological processes.
Contents
Structure and General Properties of Flavins.- Recent advances in Riboflavin Biosynthesis.- Natural Riboflavin Analogs.- A Roadmap to the Isotopolog Space of Flavocoenzymes.- Electron Transferases.- Aldonolactone Oxidoreductases.- Flavins and Flavoproteins: Applications in Medicine.- Practical Aspects on the Use of Kinetic Isotope Effects as Probes of Flavoprotein Enzyme Mechanisms.- On the in vivo Redox State of Flavin-Containing Photosensory Receptor Proteins.- Computational Spectroscopy, Dynamics and Photochemistry of Photosensory Flavoproteins.- NMR Spectroscopy on Flavins and Flavoproteins.- Solid-State NMR of Flavins and Flavoproteins.- EPR on Flavoproteins.- FTIR Spectroscopy of Flavin-Binding Photoreceptors.- Resonance Raman Spectroscopy.- Photoactivation Mechanisms of Flavin-binding Photoreceptors Revealed through Ultrafast Spectroscopy and Global Analysis Methods.- Excited State Chemistry of Flavoproteins.
