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Full Description
In addition to research and discovery, yeast surface display technology has found applications in industrial processes such as biofuel production and environmental pollutant absorption and degradation. Yeast Surface Display: Methods, Protocols, and Applications guides readers through yeast surface antibody display library and antibody engineering, yeast surface display as a tool for protein engineering, yeast surface cDNA display library construction and applications, and yeast surface display in bioassay and industrial applications. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Concise and easy-to-use, Yeast Surface Display: Methods, Protocols, and Applications aims to help accelerate the work of protein chemists, antibody engineers, molecular and cell biologists, and industrial bioengineers.
Contents
Protein Engineering and Selection Using Yeast Surface Display.- Isolation and Validation of Anti-B7-H4 scFvs from an Ovarian Cancer scFv yeast-Display Library.- Combining Phage and Yeast Cell Surface Antibody Display to Identify Novel Cell Type-Selective Internalizing Human Monoclonal Antibodies.- Yeast Display-Based Antibody Affinity Maturation Using Detergent-Solubilized Cell Lysates.- Yeast Endoplasmic Reticulum Sequestration Screening for the Engineering of Proteases from Libraries Expressed in Yeast.- T Cell Receptor Engineering and Analysis Using the Yeast Display Platform.- Epitope Specific Binder Design by Yeast Surface Display.- Applications of Yeast Surface Display for Protein Engineering.- Identification of Novel Protein-Ligand Interactions by Exon Microarray Analysis of Yeast Surface Displayed cDNA Library Selection Outputs.- Identification of Post-Translational Modification-Dependent Protein Interactions using Yeast Surface Displayed Human Proteome Libraries.- Utilizing Yeast Surface Human Proteome Display Libraries to Identify Small Molecule-Protein Interactions.- Enzyme Evolution by Yeast Cell Surface Engineering.- Electrochemical Glucose Biosensor Based on Glucose Oxidase Displayed on Yeast Surface.- Couple Binding to Catalysis - Use Yeast Cell Surface Display to Select Enzymatic Activities.- The use of Yeast Surface Display in Biofuel Cells.
