Full Description
Representing the most relevant procedures and technologies aiding the advance of the field of HPV-mediated carcinogenesis of the cervix and other anatomical regions of squamocolumnar transition, such as the anorectum, penis, and oropharynx, Cervical Cancer: Methods and Protocols compiles a detailed collection of practical chapters. The first half of the book covers HPV types, pathogenesis of cervical cancer (CxCA), prevention, and novel potential drug targets, while the second half explores pathology, genomics, modeling of CxCA, and experimental therapeutic strategies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and vital, Cervical Cancer: Methods and Protocols serves as a valuable resource to both bench scientists and clinicians who step into the realm of high-risk HPVs and CxCA for the first time or those who wish to learn novel approaches or expand their toolbox for the study of CxCA.
Contents
Evolution and Classification of Oncogenic Human Papillomavirus Types and Variants Associated with Cervical Cancer.- A Real-Time PCR Approach Based on SPF10 Primers and the INNO-LiPA HPV Genotyping Extra Assay for the Detection and Typing of Human Papillomavirus.- Replication of Human Papillomavirus in Culture.- HPV Binding Assay to Laminin-332/Integrin α6β4 on Human Keratinocytes.- Methods to Assess the Nucleocytoplasmic Shuttling of the HPV E1 Helicase and Its Effects on Cellular Proliferation and Induction of a DNA Damage Response.- Genetic Methods for Studying the Role of Viral Oncogenes in the HPV Life Cycle.- Robust HPV-18 Production in Organotypic Cultures of Primary Human Keratinocytes.- A High-Throughput Cellular Assay to Quantify the p53-Degradation Activity of E6 from Different Human Papillomavirus Types.- Retroviral Expression of Human Cystatin Genes in HeLa Cells.- Molecular Analysis of Human Papillomavirus Virus-Like Particle Activated Langerhans Cells In Vitro.- Selective Silencing of Gene Target Expression By siRNA Expression Plasmids in Human Cervical Cancer Cells.- Silencing of E6/E7 Expression in Cervical Cancer Stem-Like Cells.- Two-Step Procedure for Evaluating Experimentally Induced DNA Damage: Texas Red and Comet Assays.- Measurement of Deubiquitinating Enzyme Activity Via a Suicidal HA-Ub-VS Probe.- Immunocytochemical Analysis of the Cervical Pap Smear.- Diagnosis of HPV-Negative, Gastric-Type Adenocarcinoma of the Endocervix.- Targeting of the HPV-16 E7 Protein by RNA Aptamers.- The Use of MYBL2 as a Novel Candidate Biomarker of Cervical Cancer.- Fixation Methods for the Preservation of Morphology, RNAs, and Proteins in Paraffin-Embedded Human Cervical Cancer Cell Xenografts in Mice.- Assessment of the HPV DNA Methylation Status in Cervical Lesions.- MeDIP-on-Chip for Methylation Profiling.- Use of DBD-FISH for the Study of Cervical Cancer Progression.- A Quantitative and High-Throughput Assay of Human Papillomavirus DNAReplication.- Native Human Papillomavirus Production, Quantification, and Infectivity Analysis.- Functional Analysis of HPV-Like Particle-Activated Langerhans Cells In Vitro.- Assessment of the Radiation Sensitivity of Cervical Cancer Cell Lines.- Mouse Model of Cervicovaginal Papillomavirus Infection.- Establishment of Orthotopic Primary Cervix Cancer Xenografts.- Generation of K14-E7/∆N87ßcat Double Transgenic Mice as a Model of Cervical Cancer.
