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Full Description
Glycosaminoglycans: Chemistry and Biology emphasizes several areas of glycosaminoglycan research especially analysis and application of GAGs using a variety of approaches. Chapters focus on chemical and enzymatic synthesis of GAGs for therapeutic purposes; biophysical and biochemical methods for studying GAG-protein interactions; molecular approaches for modulating and defining GAG biosynthesis; informatics approaches for deciphering GAG code; computational approaches for establishing specific and/or non-specific interactions and genetic and biochemical tools for manipulating/visualizing glycosaminoglycan expression and studying their functions in a variety of model organisms. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Glycosaminoglycans: Chemistry and Biology serves as a valuable manual for cutting edge methodologies and practical tips to overcome any obstacles with experimentation pertaining to chemistry and biology of glycosaminoglycans.
Contents
Automated Synthesis Of Chondroitin Sulfate Oligosaccharides.- Enzymatic Synthesis Of Heparan Sulfate And Heparin.- Production Of Size-Defined Heparosan, Heparan Sulfate, and Heparin Oligosaccharides By Enzymatic Depolymerization.- Chemical Modification Of Heparin And Heparosan.- Synthesis Of Sulfur Isotope Labeled Sulfate Donor, 3'-Phosphoadenosine-5'-Phosphosulfate, For Studying Glycosaminoglycan Functions.- Preparation Of Isotope Enriched Heparan Sulfate Precursors For Structural Biology Studies.- Synthesis Of Glycosaminoglycan Mimetics Through Sulfation Of Polyphenols.- Synthesis Of Selective Inhibitors Of Heparan Sulfate And Chondroitin Sulfate Proteoglycan Biosynthesis.- Ascidian (Chordata-Tunicata) Glycosaminoglycans: Extraction, Purification, Biochemical and Spectroscopic Analysis.- Human Blood Glycosaminoglycans: Isolation and Analysis.- Chromatographic Molecular Weight Measurements For Heparin, Its Fragments And Fractions, and Other Glycosaminoglycans.- Mass Spectrometric Methods For The Analysis Of Heparin And Heparan Sulfate.- Validated Capillary Electrophoretic Assays For Disaccharide Composition Analysis Of Galactosaminoglycans In Biologic Samples and Drugs/Neutraceuticals.- Fast Screening Of Glycosaminoglycan Disaccharides By Fluorophore Assisted Carbohydrate Electrophoresis (Face): Applications To Biologic Samples And Pharmaceutical Formulations.- Capillary Electrophoretic Analysis Of Isolated Sulfated Polysaccharides To Characterize Pharmaceutical Products.- Methods For Measuring Exchangeable Protons In Glycosaminoglycans.- Heparan Sulfate Structure: Methods To Study N-Sulfation and Ndst Action.- Analysis Of Hyaluronan Synthase Activity.- A Rapid, Non-Radioactive Assay For Measuring Heparan Sulfate C-5 Epimerase Activity Using.- Aggrecan - Approaches To Study Biophysical And Biomechanical Properties.- Use Of Flow Cytometry For Characterisation And Fractionation Of Cell Populations Based On Their Expression Of Heparan SulfateEpitopes.- A Transgenic Approach To Live Imaging Of Heparan Sulfate Modification Patterns.- Informatics Tools To Advance The Biology Of Glycosaminoglycans and Proteoglycans.- Designing 'High-Affinity, High-Specificity' Glycosaminoglycan Sequences Through Computerized.- Using Isothermal Titration Calorimetry To Determine Thermodynamic Parameters Of Protein-Glycosaminoglycan Interactions.- Characterizing Protein-Glycosaminoglycan Interactions Using Solution Nmr Spectroscopy.0- Glycosaminoglycan-Protein Interaction Studies Using Fluorescence Spectroscopy.- Studying Glycosaminoglycan-Protein Interactions Using Capillary Electrophoresis.- Histochemical Analysis Of Heparan Sulfate 3-O-Sulfotransferase Expression In Mouse Brain.- Keratan Sulfate: Biosynthesis, Structures And Biological Functions.- The Sulfs: Expression, Purification And Substrate Specificity.- The Detection Of Glycosaminoglycans In Pancreatic Islets And Lymphoid Tissues.- Non-Radioactive Glycosyltransferase And Sulfotransferase Assay To Study Glycosaminoglycan Biosynthesis.- Mapping Proteoglycan Functions With Glycosidases.- Cell Substrate Patterning With Glycosaminoglycans To Study Their Biological Roles In The Central.- Analyzing The Role Of Heparan Sulfate Proteoglycans In Axon Guidance In Vivo In Zebrafish.- Murine Models In The Evaluation Of Heparan Sulfate-Based Anticoagulants.- Genetic Approaches In The Study Of Heparan Sulfate Functions In Drosophila.- Measuring Sulfatase Expression And Invasion In Glioblastoma.- Synthesis And Biomedical Applications Of Xylosides.- A Strategic Approach To Identification Of Selective Inhibitors Of Cancer Stem Cells.- Analysis Of The Heavy Chain Modification And Tsg-6 Activity In Pathological Hyaluronan Matrices.- Heparan Sulfate Modulates Slit3-Induced Endothelial Cell Migration.- Glycosaminoglycan Functionalized Nanoparticles Exploit Glycosaminoglycan Functions.- Role Of Glycosaminoglycans In Infectious Disease.- Isolation And Purification OfVersican And Analysis Of Versican Proteolysis.- Analysis Of Human Hyaluronan Synthase Gene Transcriptional Regulation and Downstream Hyaluronan Cell Surface Receptor Mobility In Myofibroblast Differentiation.
