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Full Description
The frozen-hydrated specimen is the principal element that unifies the subject of low temperature microscopy, and frozen-hydrated specimens are what this book is all about. Freezing the sample as quickly as possible and then further preparing the specimen for microscopy or microanalysis, whether still embedded in ice or not: there seem to be as many variations on this theme as there are creative scientists with problems of structure and composition to investigate. Yet all share a body of com mon fact and theory upon which their work must be based. Low-Temperature Micros copy and Analysis provides, for the first time, a comprehensive treatment of all the elements to which one needs access. What is the appeal behind the use of frozen-hydrated specimens for biological electron microscopy, and why is it so important that such a book should now have been written? If one cannot observe dynamic events as they are in progress, rapid specimen freezing at least offers the possibility to trap structures, organelles, macro molecules, or ions and other solutes in a form that is identical to what the native structure was like at the moment of trapping. The pursuit of this ideal becomes all the more necessary in electron microscopy because of the enormous increase in resolution that is available with electron-optical instruments, compared to light optical microscopes.
Contents
1. The Properties and Structure of Water.- 2. The Structure and Properties of Frozen Water and Aqueous Solutions.- 3. Sample Cooling Procedures.- 4. Cryosectioning.- 5. Low-Temperature Fracturing and Freeze-Fracture Replication.- 6. Freeze-Drying.- 7. Freeze Substitution and Low-Temperature Embedding.- 8. Low-Temperature Light Microscopy.- 9. Low-Temperature Transmission Electron Microscopy.- 10. Low-Temperature Scanning Electron Microscopy.- 11. Low-Temperature Microanalysis.- 12. Current Status of Low-Temperature Microscopy and Analysis.- References.
