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Full Description
This volume contains the lectures given at the NATO Advanced Study Institute on "Biophysics of Photoreceptors and Photomovements in Microorganisms" held in Tir renia (Pisa), Italy, in September 1990. The Institute was sponsored and mainly funded by the Scientific Affairs Division of NATO; the Physical Science Committee and the Institute of Biophysics of National Research Council of Italy also supported the School and substantially contributed to its success. It is our pleasant duty to thank these institu tions. Scientists from very different backgrounds contributed to the understanding of this fast developing field of research, which has seen considerable progress during the last years. The areas of expertise ranged from behavioral sciences, supported by sophi sticated techniques such as image analysis or laser light scattering, to spectroscopy, ap plied, in different time domains, to the study of the primary photoreactions, to electro physiology, biochemistry or molecular biology, with the aim of analyzing the various steps of the transduction chains and how they control the motor apparatus of the cells. The organisms studied covered a wide range, from bacteria to algae, fungi and other eukaryotes. Thus, the ASI represented a successful opportunity for carrying on and imple menting an interdisciplinary approach to the study of the biophysical basis of photore ception and photosensory transduction in aneural organisms, with special attention to the basic phenomena and the underlying molecular events. We hope that this book has caught the spirit in which the ASI was conceived.
Contents
Survey of Photomotile Responses in Microorganisms.- to Photosensory Transduction Chains.- Molecular Properties of Biological Light Sensors.- Photoresponses in Eubacteria.- Mechanisms and Strategies of Photomovements in Flagellates.- Mechanism and Strategies of Photomovement in Protozoa.- Image Analysis Techniques for Studying Photomovements.- Light Scattering Techniques in Studying Photoresponses.- Optical Absorption and Emission Spectroscopy of Photoreceptor Pigments.- Application of Laser Flash Photolysis to Study Photoreceptor Pigments.- Time-gated Fluorescence Spectroscopy of Photoreceptor Pigments.- Effects of Enhanced Solar Ultraviolet Radiation on Aquatic Ecosystems.- On the Trail of the Photoreceptor for Phototropism in Higher Plants.- LIAC Activity in Higher Plants.- Electrophysiology of Photomovements in Flagellated Algae.- Phototaxis and Gravitaxis in Euglena gracilis.- Photoreception in Chlamydomonas.- Processing of Photosensory Signals in Halobacterium halobium. Common Features of the Bacterial Signalling Chain and of Information Processing in Higher Developed Organisms.- Color Discriminating Pigments in Halobacterium halobium.- Absorption and Action Spectroscopy of Phoborhodopsin (Sensory Rhodopsin II).- Photoreception and Photomovements in Blepharisma japonicum.- Photoreception and Photomovements in Stentor coeruleus.- Electrophysiology and Photomovement of Stentor.- Action Spectroscopy.- Phototropism in Fungi.- High-Fluence Rate Monochromatic Light Sources, Computerized Analysis of Cell Movements, and Microbeam Irradiation of a Moving Cell: Current Experimental Methodology at the Okazaki Large Spectrograph.- Participants.
