Comprehensive Virology : 17 Methods Used in the Study of Viruses

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Comprehensive Virology : 17 Methods Used in the Study of Viruses

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  • 製本 Paperback:紙装版/ペーパーバック版/ページ数 480 p.
  • 言語 ENG
  • 商品コード 9781461566953
  • DDC分類 590

Full Description

The time seems ripe for a critical compendium of that segment of the biological universe we call viruses. Virology, as a science, having passed only recently through its descriptive phase of naming and num­ bering, has probably reached that stage at which relatively few new­ truly new-viruses will be discovered. Triggered by the intellectual probes and techniques of molecular biology, genetics, biochemical cytology, and high resolution microscopy and spectroscopy, the field has experienced a genuine information explosion. Few serious attempts have been made to chronicle these events. This comprehensive series, which will comprise some 6000 pages in a total of about 18 volumes, represents a commitment by a large group of active investigators to analyze, digest, and expostulate on the great mass of data relating to viruses, much of which is now amorphous and disjointed, and scattered throughout a wide literature. In this way, we hope to place the entire field in perspective, and to develop an invalua­ ble reference and sourcebook for researchers and students at all levels. This series is designed as a continuum that can be entered anywhere, but which also provides a logical progression of developing facts and integrated concepts.

Contents

1 Biophysical Methods in Virus Research.- 1. Introduction.- 2. Viscosity.- 3. Diffusion.- 4. Centrifugation.- 5. Sedimentation Equilibrium.- 6. Chromatography.- 7. Electrophoresis.- 8. Osmotic Pressure.- 9. Light Scatter.- 10. Theory of Electron Microscopy.- 11. References.- 2 Use of Electron Microscopy in Virology.- 1. Introduction.- 2. Specimen Methods.- 3. Survey of Results.- 4. Conclusion.- 5. References.- 3 Structural Studies of Viruses with X-Rays and Neutrons.- 1. Introduction.- 2. Physical Basis of X-Ray and Neutron Scattering.- 3. Structures of Spherical Viruses.- 4. Helicoidal Viruses.- 5. Conclusions.- 6. References.- 4 Serological Methods in the Identification and Characterization of Viruses.- 1. Introduction.- 2. Reagents.- 3. Neutralization.- 4. Agglutination.- 5. Precipitation.- 6. Tests with Labeled Antibodies.- 7. Complement Fixation.- 8. Immunoelectron Microscopy.- 9. Applications of Serological Methods.- 10. References.- 5 Chemical Modification of Viruses.- 1. Introduction.- 2. Reagents Used for Virus Modification.- 3. Modifications for Specific Purposes.- 4. Conclusions.- 5. References.- 6 Photobiology of RNA Viruses.- 1. Introduction.- 2. Plant Viruses.- 3. RNA Bacteriophages.- 4. Animal Viruses.- 5. Conclusion.- 6. References.- 7 The Photobiology of the DNA Viruses ?,T4, and ?X174.- 1. Introduction.- 2. ? Phage.- 3. UV Effects on Bacteriophage T4 (T2 and T6).- 4. UV Effects on ?X174.- 5. Conclusion.- 6. References.

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