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Full Description
This concise, truncated version of Nagy, Varghese and Agarwal's Practical Manual of In Vitro Fertilization is comprised of select clinical chapters for a portable, affordable and up-to-date resource. Gamete Assessment, Selection and Micromanipulation in ART covers a variety of topics, including:
- Human oocyte evaluation
- Sperm evaluation and selection
- Micromanipulators and micromanipulation
- Biopsy procedures on oocytes and embryos
- Molecular insights in IVF
Practical for both clinicians and researchers alike, Gamete Assessment, Selection and Micromanipulation in ART brings together all of the need-to-know information about these important topics in reproductive medicine.
Contents
Part I: Human Oocyte Evaluation in IVF.- Assessment of Oocyte Quality.- Polarization Microscopy.- Cumulus Cell Gene Expression in Assessment of Oocyte Quality.- Part II: Sperm Evaluation and Selection.- Sperm Assessment: Traditional Approaches and Their Indicative Value.- Sperm Assessment: Novel Approaches and Their Indicative Value.- Intracytoplasmic Morphologically Selected Sperm Injection.- Sperm Testing and ICSI Selection by Hyaluronic Acid Binding: The Hyaluronic Acid-Coated Glass Slide and Petri Dish in Andrology and IVF Laboratories.- Electrophoretic Sperm Separation.- Magnetic-Activated Cell Sorting of Human Spermatozoa.- Polscope-Based Sperm Selection.- Part III. Micromanipulators and Micromanipulation.- Hydraulic Manipulators for ICSI.- Research Instruments Micromanipulators.- Eppendorf Micromanipulator: Setting Up and Operation of Micromanipulators.- The Leica Microsystem IMSI System.- Automated Robotic Intracytoplasmic Sperm Injection.- Oocyte Treatment and Preparation for Microinjection.- Mechanism of Human Oocyte Activation during ICSI and Methodology for Overcoming Low or Failed Fertilization.- Livestock Production via Micromanipulation.- Assisted Hatching in IVF.- Part IV: Biopsy Procedures on Oocytes and Embryos.- Polar Body Biopsy.- Cleavage-Stage Embryo Biopsy.- Embryo Biopsy for PGD: Current Perspective.- Microarrays and CGH for PGD of Chromosome Abnormalities and Gene Defects.- Part V: Molecular Insights.- The Role of Mitochondria in the Establishment of Developmental Competence in Early Human Development.- Nuclear and Cytoplasmic Transfer: Human Applications and Concerns.- Cytoskeletal Architecture of Human Oocytes with Focus on Centrosomes and Their Significant Role in Fertilization.- Molecular Mining of Follicular Fluid for Reliable Biomarkers of Human Oocyte and Embryo Developmental Competence.
