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Full Description
It has always been the policy of SUBCELLULAR BIOCHEMISTRY to be flexible in the size of its articles. In some cases the author may wish to deal with a relatively narrow aspect of his subject and suggestions that he enlarge the article would not necessarily be welcomed. On the other hand, there are occasions when the topic demands a large canvas. It could be argued that the present facilities available in the biochemical literature are inadequate for those who wish to develop an argument in extenso. Short of publishing a book on the subject (a course of action that would raise all sorts of questions of marketability, presentation, etc. ), an author with an article that passes the 1- page barrier could well find great difficulty in finding an outlet for his work. We are therefore very happy to include in this volume an extensive and erudite account of electron microscopy by H. Plattner and H. P. Zingsheim. The article deals initially with all aspects of modern electron microscopy as applied to biological problems. For every basic technique used, the physical background and theory is clearly explained and extensive references are given to the various adaptations and modifications of each technique. The article is richly endowed with diagrams, tables, and sample electron micrographs and should be extremely useful both to the "cognoscenti" and to those first entering the technically demanding but nevertheless fascinating field of electron micros copy.
Contents
1 Electron Microscopic in Cellular and Molecular Biology.- 1 Introduction.- 2 The Electron Microscope.- 3 Preparation and Analysis—Structural Aspects.- 4 Preparation and Analysis—Functional Aspects.- 5. Molecular Electron Microscopy.- 6. Units and Conventions.- 7. Perspectives.- References.- 2 Chloroplast Protein Synthesis: Principles and Problems.- 1 Introduction.- 2. Possible Principles Governing Nuclear-Chloroplast Interactions.- 3. The Nucleocytoplasmic Origin of the Majority of Chloroplast.- 4. The Post-Translational Import of Polypeptides into.- 5. The Essential Contribution of the Chloroplast Genome.- 6. The Confinement of Chloroplast DNA-Encoded Molecules.- 7. The Stimulatory Role of Light.- References.- 3 Thesaurisomes, A Novel Kind of Nucleoprotein Particle.- 1 Introduction.- 2. Discovery of the Thesaurisomes.- 3. Composition of Thesaurisomes.- 4. Comparison Between the 7S and 42S Particles.- 5. Relationship Between RNA and Protein in Thesaurisomes.- 6. Intracellular Location of Thesaurisomes.- 7. Comparison Between Thesaurisomes and Ribosomes.- 8. Relationship Between Thesaurisomes and Ribosomes in the Oocyte.- 9. State of Acylation of tRNA in Thesaurisomesz.- 10. Reconstruction of the Thesaurisomes.- 11. Occurrence and Role of Thesaurisomes.- 12. Thesaurisomes as Objects for Structural Studies.- 4 Platelet Phospholipid Asymmetry and its Significance in Hemostasis.- 1 Introduction.- 2. Aspects of Platelet Structure and Function.- 3. Membrane Lipid Asymmetry.- 4. Mechanism and Significance of Changes in Membrane Phospholipid Orientation.- 5. Conclusion.- References.- 5 The Diversity of Function and Structure of Cellular Membranes.- 1. The Approach.- 2. Mitochondria.- 3. Photoreceptor Cell Outer Segment Disks.- 4. A Plasma Membrane.- 5. Conclusions.- 6. ANote on the Danielli-Davson Membrane Model.- References.- Some Recent Books in Cell Biochemistry and Biology.- 1. Evolution and Development.- 2. Plant Cell Biochemistry.- 3. Miscellaneous Topics.
