Full Description
In recent years excellent progress has been the study of these disorders in the future. made in the development of improved Recently freeze fracture techniques have cytochemical and ultrastructural tech- also been employed in the study of normal niques for electron microscopy in hematol- and leukemic lymphocytes and much new ogy and in particular in leukemia and information concerning leukemic cell lymphoma. Subtypes of these disorders, membranes may be obtained from the use which are essentially classified according to of this technique in the future. cell phenotype and the degree of matura- The purpose of this book is to demon- tion, can be well defined today using a strate the utility of these techniques in combination of immunological methods, defining leukemic cell types and to illus- light microscopy, cytochemistry, and elec- trate the potential of some of the newer tron microscopy.
Ultrastructural cyto- ultrastructural methods, including trans- chemistry is now utilized routinely in mission and scanning immuno electron leukemia in most major centers today and microscopy, SEM cytochemistry, and can also be performed using scanning elec- freeze fracture techniques, in the study of tron microscopy (SEM), as described in leukemia. Advances in cytochemical tech- this book. Immuno electron microscopy niques and their use in the definition of used with or without simultaneous ultra- nonlymphoid cells and in the classification structural cytochemistry can also be em- of lymphoid subtypes are clearly demon- ployed in the accurate classification of strated in this book.
Contents
I. Cytochemistry, Light Microscopy.- 1. Contribution of Cytochemistry in Leukemia.- 2. Cytochemical Distribution of 5-Nucleotidase Activity in Human Leukemic cells.- 3. Significance of Improved Cytochemical Methods for Hemoprotein Enzymes in Diagnosis and Classification of Leukemia.- II. Transmission Electron Microscopy, Ultrastructural Cytochemistry.- 4. Pathology of Neutrophil Granules: Selective Abnormalities of Azurophil and Specific Granules of Human Neutrophilic Leukocytes.- 5. Review of the Ultrastructure and Cytochemistry of Megakaryoblastic Leukemia.- 6. The Ultrastructure of Monocytic Leukemia.- 7. Blastic Transformation of Chronic Granulocytic Leukemia and other Myeloproliferative Disorders: A study of 26 cases Emphasizing the Importance of Cytochemistry and Ultrastructure in Defining the Cell Phenotype.- 8. Ultrastructural Observations in Therapy-Related Panmyelosis.- 9. A comparison of the Ultrastructure of Secondary Leukemias with de Novo Acute non-Lymphoblastic Leukemia and Blast Crisis of Chronic Granulo cytic Leukemia.- 10. Acute Lymphoblastic Leukemia.- 11. Acute Lymphoblastic Leukemia: Cytochemistry and Ultrastructure with Emphasis on the Hand Mirror Variant.- 12. Prolymphocytic Leukemia of B- and T-cell Types: Morphological Differences by Light and Electron Microscopy.- 13. Ultrastructural Analysis of B- and T-lymphocyie Subsets with Immunogold Technique: Its relevance to the Study of Chronic Lymphocytic Leukemias.- 14. Cytochemistry and Ultrastructure of T-cell Chronic Lymphocytic Leukemia.- 15. Fine structure of leukemic T- and B-cells, with Special Reference to Adult T-cell Leukemia in Japan.- 16. Ultrastructure of Adult T-cell Leukemia in Japan.- 17. Plasma Cell Leukemia: Ultrastructural Aspects in Diagnosis.- 18. Sézary Syndrome: Cytochemistryand Ultrastructure.- 19. Hairy Cell Leukemia: Cytochemistry and infrastructure (TEM) in Diagnosis.- 20. Ultrastructure of the Leukemic Phase in Non-Hodgkin's Lymphoma.- III. Transmission Immuno Electron Microscopy.- 21. Immuno Electron Microscopic Observations in Human B-cell Leukemias.- IV. Freeze-Fracture, Ultrastructure.- 22. Principles of Freeze-Fracture Technique and Implications in Studies of Normal and CLL Lymphocytes.- V. Scanning Electron Microscopy.- 23. Surface Morphology of Leukemic Cells as Seen by Scanning Electron Microscopy: Interpretation and Attempted Correlation with Cell Type in 212 Cases of Leukemia.- 24. Scanning Electron Microscopy and Leukemia-Lymphoma Characterization.- 25. Scanning Electron Microscopy of Hairy Cells from 15 Patients with Hairy Cell Leukemia: Morphologic Subtypes.- VI. Scanning Immuno-Electron Microscopy.- 26. Immuno Scanning Electron Microscopy of Human Leukemic Cells: A Study of 50 Cases of Leukemia and 14 Established Leukemia-lymphoma Cell Lines.- VII. Scanning Electron Microscopy, Cytochemistry.- 27. Scanning Electron Microscopic Cytochemistry of Leukemic Cells.- VIII. Ultrastructure. In-Vitro Studies of Leukemia.- 28. Ultrascructure of Cultured Leukemia Cell Lines..- 29. Viruses and Human Leukemia in Vitro..
