Full Description
Procedure Manual for the Diagnosis of Intestinal Parasites is the definitive resource for individuals involved in the collection, preparation, and examination of fecal specimens for microscopic diagnosis of intestinal parasitic infections. The book points out the stages of parasites possibly found in a fecal specimen, how to find them, and how to identify them. Specific details on how to effectively use the microscope for parasitic diagnosis are included. This information is missing from most texts and manuals of this kind.Photomicrographs and original drawings of the various stages and forms of parasites and eggs are used extensively throughout the text, with the photomicrographs printed to a standard scale for easy comparison. More than 400 illustrations in all are included. Biological keys for intestinal amoebae and eggs of various species of helminths are provided.This book is an essential reference for teachers of diagnostic parasitology and their students, physicians who order fecal examinations for intestinal parasites, nurses or health workers who handle or prepare the specimens for the laboratory, and technologists who receive, process, and examine the specimens.
Contents
IntroductionSurvival of Trophozoites and Cysts in Unpreserved Fecal SpecimensCollecting/Preserving SolutionsThe Use of Collecting/Preserving SolutionsThe Quality of Preserved Eggs and ParasitesBackground of the MIF-Type Collecting/Preserving SolutionsEnvironmental ConsiderationsApplication of Collecting/Preserving MethodsThe SpecimenFecal Specimens in the Clinical LaboratoryFactors Having an Impacting on Specimen CollectionProcedure for Specimen Collection for ParasitologyFactors Having an Impact on Specimen PreparationWritten Instructions for Specimen Collection.Instructions for Collecting Fecal SpecimensThe Microscope and Examination of the SpecimenThe Microscope and MicroscopyUsing Their Refractive Index to Find Parasites and EggsEyepiece MicrometerSelection of LensesPreparing Wet-Mount Slides from the Preserved Specimen for Microscopic ExaminationConcentration MethodsIntroductionUse of the Centrifuge for Concentration MethodsFlotation MethodsSedimentation MethodsStains and Staining MethodsStains and Staining MethodsPreparing Permanently Stained Fecal FilmsPreparing Fecal Films for Permanent StainingStaining Methods for Preparing Permanently Stained Fecal Films from Specimens Fixed in PVAStaining Methods for Preparing Permanently Stained Fecal Films from Specimens Fixed in SAF, PIF, or MIFReusing and Changing Solutions in the Staining SetupApplication of MethodsComparison of Collecting/Preserving Solutions.Selecting Methods for the LaboratorySpecial MethodsAcid-Fast StainsPreparing Temporary Wet MountsEgg Count ProceduresPost-Treatment ExaminationsIntroduction to the ParasitesParasites Identified by Examining Fecal PreparationsHelminth Parasites in GeneralProtozoan Parasites in GeneralSource of Infection and Modes of TransmissionThe HelminthsDiagnosis of Helminth InfectionsTrematode EggsCestode EggsNematode EggsNematode JuvenilesIntroduction to the "Key to Helminth Eggs Found in Feces"Photomicrographs of Helminth EggsKey to Helminth Eggs Found in FecesThe ProtozoaStructure of the Intestinal ProtozoaStructure of the Intestinal AmoebaeStructure of the Intestinal FlagellatesStructure of the Intestinal CiliatesStructure of the Intestinal CoccidiaThe Intestinal AmoebaeIntroduction to the "Key for Differentiating Species of Intestinal Amoebae in Fecal Specimens Preserved in PIF or MIF"Key for Differentiating Species of Intestinal Amoebae in Specimens Preserved in PIF or MIFThe Intestinal FlagellatesThe Intestinal CiliatesThe Intestinal CoccidiaProtozoa of Uncertain Taxonomic PositionPhotomicropgraphs of Intestinal ProtozoaNonhuman Parasites and Structures that Mimic ParasitesStructures Resembling ProtozoaStructures Resembling Helminth EggsSpurious Infections with Eggs and Cysts of AnimalsAppendices Technical InformationGlossaryReferencesIndex
