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Full Description
Since most therapeutic efforts have been predominantly focused on pharmaceuticals that target proteins, there is an unmet need to develop drugs that intercept cellular pathways that critically involve nucleic acids. Progress in the discovery of nucleic acid binding drugs naturally relies on the availability of analytical methods that assess the efficacy and nature of interactions between nucleic acids and their putative ligands. This progress can benefit tremendously from new methods that probe nucleic acid/ligand interactions both rapidly and quantitatively.
A variety of novel methods for these studies have emerged in recent years, and Methods for Studying DNA/Drug Interactions highlights new and non-conventional methods for exploring nucleic acid/ligand interactions. Designed to present drug-developing companies with a survey of possible future techniques, the book compares their drawbacks and advantages with respect to commonly used tools. Perhaps more importantly, this book was written to inspire young scientists to continue to advance these methods into fruition, especially in light of current capabilities for assay miniaturization and enhanced sensitivity using microfluidics and nanomaterials.
Contents
CLASSICAL TECHNIQUES: Using Spectroscopic Techniques to Examine Drug-DNA Interactions. Probing DNA and RNA Interactions with Biogenic and Synthetic Polyamines: Models and Biological Implications. EMERGING TECHNIQUES: Mass Spectrometry-Based Techniques for Studying Nucleic Acid/Small Molecule Interactions.
Real-Time Monitoring of Nucleic Acid Interactions with Biosensor-Surface Plasmon Resonance. Studying Aptamer/Ligand Interactions Using Fluorescence Correlation Spectroscopy. Studying Nucleic Acid - Drug Interactions at the Single Molecule Level Using Optical Tweezers. Fluorescent Nucleoside Analogues for Monitoring RNA-Drug Interactions. Atomic Force Microscopy Investigation of DNA-Drug Interactions. Characterizing RNA-Ligand Interactions Using 2-Dimensional Combinatorial Screening. EPR Spectroscopy for the Study of RNA-Ligand Interactions. Electrochemical Approaches to the Study of DNA-Drug Interactions. Nanopore Ion Microscope for Detecting Nucleic Acid/Drug Interactions. A Primer for Relaxation Kinetic Measurements. DNA-Drug Interactions: A Theoretical Perspective. Computational Studies of RNA Dynamics and RNA-Ligand Interactions.
All chapters include case studies, background and basics, prospects and outlook, conclusions, and references
