Full Description
This volume discusses the current models and latest methodologies used to explore key questions in transfusion medicine. The chapters in this book cover topics such as mouse models of platelet alloimmunization refractoriness and immune thrombocytopenia; analysis of biotinylated red cell blood cells following transfusion; impact of intravascular hemolysis on erythropoiesis in sickle cell disease; western blot detection of blood group A and B antigens; and the role of marginal zone B cells in FVIII inhibitor development. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Thorough and comprehensive, Methods in Transfusion Medicine is a valuable resource for all researchers interested in learning more about this field and leading us closer to optimal transfusion practice.
Contents
The Immunology of Transfusion Medicine: Past, Present, and Future.- Characterization of Platelet and Red Blood Cell Extracellular Vesicles by Spectral Flow Cytometry.- Mouse Models of Platelet Alloimmunization and Refractoriness.- Generation and Use of an Alloantigen-Specific Preclinical Humanized Mouse Model of Fetal and Neonatal Alloimmune Thrombocytopenia.- Mouse Model of Hemolytic Disease of the Fetus and Newborn.- Anti-PF4/Heparin Antibody Production in a Mouse Immunization Model.- Microscopy-Based Methods for Measuring Platelet and Red Blood Cell Phagocytosis.- Miniaturized In Vitro Transfusion Model.- Mouse Models of Immune Thrombocytopenia.- Methods to Examine Complement Activation by HIT Immune Complexes.- Cloning of Antibodies from Patients with Heparin-Induced Thrombocytopenia.- A Detailed Protocol to Induce and Treat Passive Immune Thrombocytopenia in Mice.- Platelet Transfusion in Murine Models of Neonatal Thrombocytopenia.- Quantification of Human Platelet Clearance In Vitro and In Xenotransfused Animals.- Tracking and Testing Transfused Versus Endogenous Platelets with Biotin or Human Leukocyte Antigen.- Analysis of Biotinylated Red Cell Blood Cells Following Transfusion.- Examination of Antigen-Specific B Cell Responses to RBC Transfusion.- Determining the Binding Affinity in Monoclonal Antibody-Alloantigen Interactions.- Modeling the Application of Chimeric Antigen Receptor T Cell Therapy to Eliminate Alloantibodies in Mice.- Examination of Class I HLA-Fc Fusion Proteins in Targeting Cognate Antibody-Producing Cells.- Isolation and Characterization of Mesenchymal Stem Cells from Human Umbilical Cord Tissue.- Prolonging the Lifespan of Neutrophils for Granulocyte Transfusion in Neutropenic Mice with Bacterial Pneumonia.- Mouse Models of Transfusion-Related Acute Lung Injury (TRALI).- Methodological Approaches to Study Transfusion-Related Acute Lung Injury (TRALI): From Complement Activation to In Vivo Mouse Model.- High-Throughput Metabolomics in Transfusion Medicine.- Assessing the Impact of Intravascular Hemolysis on Erythropoiesis in Sickle Cell Disease.- Murine Models of Transfusion-Induced Red Blood Cell Alloimmunization.- Establishing, Maintaining, and Cryopreserving In Vitro Cultures of the Asexual Stages of Babesia divergens.- Building the Toolkits to Examine the Expression and Associated Regulation of RBC Alloantigens.- Use of Microbial Microarrays to Define Antibody Specificity.- Production of Induced Pluripotent Stem Cell-Derived Red Blood Cells.- Red Blood Cell Storage and Post-Transfusion Recovery.- The Use of Bioengineered Induced Pluripotent Stem Cell-Derived Human Megakaryocytes to Diagnose and Characterize Platelet Allo-, Auto- and Iso-Immune Disorders.- Defining the Specificity of Blood-Group Binding Lectins through Microarray Analysis.- Characterization of Antigen Specific CD4+ T Cell Responses to the Transfusion of Red Blood Cells.- Defining the Role of Dendritic Cells in Red Blood Cell Alloimmunization.- Examination of the Immunological Role of Toll-Like Receptor 9 on Red Blood Cells.- Examining Inflammatory Effects of Reticulocyte Erythrophagocytosis in Human Macrophage Cultures.- Anemia and Transfusion in Preclinical Models of Neonatology.- Expression and Characterization of Blood Group Binding Lectins.- Acute Incompatible Red Blood Cell Transfusion in Mice.- In Vitro Phagocytosis Assays.- Analysis of Galectin Binding to Blood Group Expressing Bacteria.- Western Blot Detection of Blood Group A and B Antigens.- Flow Cytometric Examination of Galectin Binding to Red Blood Cells.- Analysis of Erythrocyte Membrane Alloantigens.- Extraction and Quantification of Skin Immune Cells and Skin Lipids.- Defining the Role of Marginal Zone B Cells in RBC Alloimmunization.- A Flow Cytometric Methods of Determining CD71+ Erythroid Cells in Neonatal Whole Blood.- Defining the Role of Marginal Zone B Cells in FVIII Inhibitor Development.
