Xenopus : Methods and Protocols (Methods in Molecular Biology)

Beck, Caroline W. (EDT)

Humana（2026/07発売）

• 製本 Hardcover:ハードカバー版
• 言語 ENG
• 商品コード 9781071653593

Full Description

This detailed volume brings together protocols spanning classical and modern approaches to using Xenopus laevis and/or Xenopus tropicalis in scientific research. From maintaining healthy colonies and raising tadpoles to protocols for embryo culture, microinjection, CRISPR and base editing, and transgenesis, this book showcases the versatility of this tractable model in the study of developmental biology, organogenesis, human disease, and regeneration. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls.

Authoritative and up-to-date, Xenopus: Methods and Protocols serves as an ideal guide for researchers both new to, and familiar with, this adaptable model organism.

Chapters 1, 3, 9, 11, and 20 are available open access under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License via link.springer.com.

Contents

Best Practices in Xenopus Housing, Husbandry, and the 3Rs.- Producing Xenopus Eggs and Embryos.- Xenbase: A Guide to the Xenopus Frog Genetics and Genomics Knowledgebase.- mRNA Microinjection for Gene Expression Studies in Xenopus laevis and Xenopus tropicalis.- CRISPR-Mediated Gene Editing: Generating Gene Knockouts or Precision Base Changes in F0 Xenopus.- Generation of Xenopus Transgenic Lines Using I-SceI.- Xenopus laevis Egg Extracts.- Cryogenic Preservation of Xenopus Sperm.- Adeno-Associated Virus (AAV) for Tracking Neural Development and Connectivity in Xenopus.- Whole Mount In Situ Hybridization in Xenopus.- Fluorescent In Situ mRNA Hybridization (FISH) Using Hybridization Chain Reaction (HCR) in Xenopus Cryosections.- Immunolocalization in Xenopus.- In Vivo Widefield Fluorescence Ca2+ Imaging of Xenopus Tadpole Brains.- Blood-Brain Barrier Disruption and Edema in Xenopus: Methods for Analysis.- Automated Behavioral Tracking and Analysis of Xenopus Tadpoles.- Working Memory Y-Maze Test for Xenopus Tadpoles.- Producing Gynogenetic Embryos in Xenopus tropicalis.- Biomechanical Studies with Xenopus Animal Caps: A 3D-Printed Mechanical Bracket for Maintaining Tissue Stretch During Immunofluorescence Analysis.- Direct Observation and Measurement of Membrane Tension in Xenopus laevis Animal Cap Explants Using the Mechanosensitive Dye, Flipper-TR.- Spinal Cord Regeneration in Xenopus.- Retinal Degeneration and Regeneration in Xenopus laevis Tadpoles.- Determining Sex in Xenopus laevis Tadpoles.- Bone and Cartilage Staining for Skeletal Preparations.