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Full Description
This detailed volume covers diverse aspects of stem cell research and exemplifies some of the developments and improvements in the field. The book explores how to regulate and manipulate stem cells appropriately in vitro, allowing for the generation of very early embryo-like structures, known as blastoids, opening up opportunities for analyzing and delineating the very early steps in embryo development under controlled in vitro conditions. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Stem Cell-Based Human Embryo Models: Methods and Protocols serves as an ideal guide for researchers looking to drive rapid innovations in the study of early embryonic development.
Contents
Human Stem Cell-Based Embryo Model Research: Ethics and Regulations.- From Single Stem Cells to an In Vitro Model of the Post-Implantation Human Embryo: A Step-by-Step Guide.- Generating Human Blastoids Following Transient Naïve Resetting of Primed Pluripotent Stem Cells.- Generation of Blastoids from Human Naïve Pluripotent Stem Cells.- Use of miR-200-Mediated Reprogramming and Mechanical Cues to Generate Human Blastocyst Models.- Generation of the Non-Integrated Human Bilaminar Embryo Model (Bilaminoid) from Naïve Pluripotent Stem Cells.- Modeling Somitogenesis and Axial Development In Vitro with Axioloids.- From Stem Cells to Post-Gastrulation Amnioids (PGAs): A Protocol to Model Human Extra-Embryonic Development.- Interaction Between Pluripotent Stem Cells and Amniotic Epithelium for the In Vitro Study of Human Embryogenesis.- Extended In Vitro Culture of Human Embryos.- Generation of Human Naïve Pluripotent Stem Cell-Derived Blastoids in Thermoformed Microwell Platforms.- Microfluidic On-Chip Assay for Quantifying Blastoid Adhesion to Endometrial Epithelium.- Linking Single-Cell Dynamics to Cell Fate in Differentiating hPSCs.- Application of CRISPR-Based Epigenome Editing Tools for Engineering Programmable Embryo Models.- Direct Reprogramming of Human Fibroblasts into Fully Functional Trophoblast Stem Cells.- 3D In Vitro Culture of Early Mouse Embryos and Trophoblast Tissue Explants.- Generation of Porcine Blastoids from Embryonic Stem Cells.- A Deep Learning Approach to Assessing Cell Identity in Stem Cell-Based Embryo Models.- An Optimized Protocol for the Extended Culture of Gastruloids from Mouse Embryonic Stem Cells.- Optimization of Gastruloid Pre-Culture Conditions for Reproducible Germ Layer Composition.- Microfluidic Device Manufacturing by Light-Based 3D Printing for Organoid Vascularization.- Engineering Synthetic PEG Hydrogels to Model Peri-Implantation Epiblast Morphogenesis with Tunable Biophysical Properties.
